Isocudraxanthone K Induces Growth Inhibition and Apoptosis in Oral Cancer Cells via Hypoxia Inducible Factor-1α
Joint Authors
Kim, Eun-Cheol
Kim, Youn-Chul
Kang, Soo-Kyung
Shin, Mee-Ran
Lee, Young-Man
Auh, Q-Schick
Lee, Hwa-Jeong
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-03
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Isocudraxanthone K (IK) is a novel, natural compound from a methanol extract of the root bark of Cudrania tricuspidata.
It has not been shown previously that IK possessed antitumor activity.
We investigated the antitumor effects and molecular mechanism of IK and related signal transduction pathway(s) in oral squamous cell carcinoma cells (OSCCCs).
The MTT assay revealed that IK had an antiproliferative effect on OSCCCs, in a dose- and time-dependent manner.
IK induced apoptosis in OSCCCs, as identified by a cell-cycle analysis, annexin V-FITC and propidium iodide staining, and the nuclear morphology in cell death.
IK caused time-dependent phosphorylation of Akt, p38, and ERK (extracellular signal-regulated kinase).
In addition, IK increased the cytosolic to nuclear translocation of nuclear factor-κB (NF-κB) p65 and the degradation and phosphorylation of IκB-α in HN4 and HN12 cells.
Furthermore, IK treatment downregulated hypoxia-inducible factor 1α (HIF-1α) and its target gene, vascular endothelial growth factor (VEGF).
Cobalt chloride (CoCl2), a HIF-1α activator, attenuated the IK-induced growth-inhibiting and apoptosis-inducing effects, and blocked IK-induced expression of apoptosis regulatory proteins, such as Bax, Bcl-2, caspase-3, caspase-8, and caspase-9, and cytochrome c.
Collectively, these data provide the first evidence of antiproliferative and apoptosis-inducing effects of IK as a HIF-1α inhibitor and suggest it may be a drug candidate for chemotherapy against oral cancer.
American Psychological Association (APA)
Shin, Mee-Ran& Lee, Hwa-Jeong& Kang, Soo-Kyung& Auh, Q-Schick& Lee, Young-Man& Kim, Youn-Chul…[et al.]. 2014. Isocudraxanthone K Induces Growth Inhibition and Apoptosis in Oral Cancer Cells via Hypoxia Inducible Factor-1α. BioMed Research International،Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-509401
Modern Language Association (MLA)
Shin, Mee-Ran…[et al.]. Isocudraxanthone K Induces Growth Inhibition and Apoptosis in Oral Cancer Cells via Hypoxia Inducible Factor-1α. BioMed Research International No. 2014 (2014), pp.1-14.
https://search.emarefa.net/detail/BIM-509401
American Medical Association (AMA)
Shin, Mee-Ran& Lee, Hwa-Jeong& Kang, Soo-Kyung& Auh, Q-Schick& Lee, Young-Man& Kim, Youn-Chul…[et al.]. Isocudraxanthone K Induces Growth Inhibition and Apoptosis in Oral Cancer Cells via Hypoxia Inducible Factor-1α. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-509401
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-509401