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Characterization of Recombinant Dengue-2 Virus Derived from a Single Nucleotide Substitution in the 5′ Noncoding Region
Joint Authors
Leardkamolkarn, Vijittra
Sirigulpanit, Wipawan
Kinney, Richard M.
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-03-22
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Variants of wild-type dengue serotype 2 (DEN-2) virus containing nucleotide substitutions at positions 14, 15, or 57 in the 5′ NCR were constructed by PCR-mediated site-directed mutagenesis.
All three viruses containing a single point substitution demonstrated attenuation phenotype as evidenced by decreases replication and plaque size in cell culture assay.
All three variants were less neurovirulent in newborn mice compared to the wild type.
The mutants were immunogenic in adult mice immunogenicity and maintained stable replication characteristics following passage in mice.
The variant viruses were competent for replication in Aedes aegypi mosquito vector, albeit at lower levels of infection and dissemination in the mosquito than the wild-type Den-2 16681 virus.
Although all of the viruses, including the wild type, were found transmissible in mosquito life cycles, they were found subsequentially decreased in efficiency of infection, transmission, and dissemination rates along the mosquito generations and all of them remained genetically stable.
American Psychological Association (APA)
Leardkamolkarn, Vijittra& Sirigulpanit, Wipawan& Kinney, Richard M.. 2010. Characterization of Recombinant Dengue-2 Virus Derived from a Single Nucleotide Substitution in the 5′ Noncoding Region. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-509402
Modern Language Association (MLA)
Leardkamolkarn, Vijittra…[et al.]. Characterization of Recombinant Dengue-2 Virus Derived from a Single Nucleotide Substitution in the 5′ Noncoding Region. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-8.
https://search.emarefa.net/detail/BIM-509402
American Medical Association (AMA)
Leardkamolkarn, Vijittra& Sirigulpanit, Wipawan& Kinney, Richard M.. Characterization of Recombinant Dengue-2 Virus Derived from a Single Nucleotide Substitution in the 5′ Noncoding Region. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-509402
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-509402