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Proteomics and Metabolomics for In Situ Monitoring of Wound Healing
Joint Authors
von Bergen, Martin
Rammelt, Stefan
Förster, Yvonne
Schmidt, Johannes
Eckelt, Uwe
Hempel, Ute
Weißflog, Anne
Baumann, Sven
Gao, Wenling
Schulz, Matthias C.
Kalkhof, Stefan
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-04
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Wound healing of soft tissue and bone defects is a complex process in which cellular differentiation and adaption are regulated by internal and external factors, among them are many different proteins.
In contrast to insights into the significance of various single proteins based on model systems, the knowledge about the processes at the actual site of wound healing is still limited.
This is caused by a general lack of methods that allow sampling of extracellular factors, metabolites, and proteins in situ.
Sampling of wound fluids in combination with proteomics and metabolomics is one of the promising approaches to gain comprehensive and time resolved data on effector molecules.
Here, we describe an approach to sample metabolites by microdialysis and to extract proteins simultaneously by adsorption.
With this approach it is possible (i) to collect, enrich, and purify proteins for a comprehensive proteome analysis; (ii) to detect more than 600 proteins in different defects including more than 100 secreted proteins, of which many proteins have previously been demonstrated to have diagnostic or predictive power for the wound healing state; and (iii) to combine continuous sampling of cytokines and metabolites and discontinuous sampling of larger proteins to gain complementary information of the same defect.
American Psychological Association (APA)
Kalkhof, Stefan& Förster, Yvonne& Schmidt, Johannes& Schulz, Matthias C.& Baumann, Sven& Weißflog, Anne…[et al.]. 2014. Proteomics and Metabolomics for In Situ Monitoring of Wound Healing. BioMed Research International،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-509423
Modern Language Association (MLA)
Kalkhof, Stefan…[et al.]. Proteomics and Metabolomics for In Situ Monitoring of Wound Healing. BioMed Research International No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-509423
American Medical Association (AMA)
Kalkhof, Stefan& Förster, Yvonne& Schmidt, Johannes& Schulz, Matthias C.& Baumann, Sven& Weißflog, Anne…[et al.]. Proteomics and Metabolomics for In Situ Monitoring of Wound Healing. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-509423
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-509423