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Immobilization of HRP Enzyme on Layered Double Hydroxides for Biosensor Application
Joint Authors
Baccar, Zouhair M.
Hafaiedh, Imène
Source
International Journal of Electrochemistry
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-28
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
We present a new biosensor for hydrogen peroxide (H2O2) detection.
The biosensor was based on the immobilization of horseradish peroxidase (HRP) enzyme on layered double hydroxides- (LDH-) modified gold surface.
The hydrotalcite LDH (Mg2Al) was prepared by coprecipitation in constant pH and in ambient temperature.
The immobilization of the peroxidase on layered hybrid materials was realized via electrostatic adsorption autoassembly process.
The detection of hydrogen peroxide was successfully observed in PBS buffer with cyclic voltammetry and the chronoamperometry techniques.
A limit detection of 9 μM of H2O2 was obtained with a good reproducibility.
We investigate the sensitivity of our developed biosensor for H2O2 detection in raw milk.
American Psychological Association (APA)
Baccar, Zouhair M.& Hafaiedh, Imène. 2011. Immobilization of HRP Enzyme on Layered Double Hydroxides for Biosensor Application. International Journal of Electrochemistry،Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-509428
Modern Language Association (MLA)
Baccar, Zouhair M.& Hafaiedh, Imène. Immobilization of HRP Enzyme on Layered Double Hydroxides for Biosensor Application. International Journal of Electrochemistry No. 2011 (2011), pp.1-5.
https://search.emarefa.net/detail/BIM-509428
American Medical Association (AMA)
Baccar, Zouhair M.& Hafaiedh, Imène. Immobilization of HRP Enzyme on Layered Double Hydroxides for Biosensor Application. International Journal of Electrochemistry. 2011. Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-509428
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-509428