Immobilization of DNA Aptamers on Polyester Cloth for Antigen Detection by Dot Blot Immunoenzymatic Assay (Aptablot)
Joint Authors
Blais, Burton
Smiley, Sally
DeRosa, Maria C.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-30
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
A simple dot blot immunoenzymatic assay system was developed using polyester cloth coated with an oligo-DNA aptamer to provide a high-affinity macroporous surface for the efficient capture of a model protein analyte (thrombin) in complex sample matrices such as foods.
Bound thrombin was detected immunoenzymatically using a peroxidase-linked antithrombin antibody and a chromogenic substrate.
A unique feature of this approach, which we have termed “aptablot,” is the facile immobilization of DNA aptamers on the polyester surface by cross-linking with a brief exposure to ultraviolet light, and the simple assay format obviating the need for specialized instruments.
The assay principle described herein should be broadly applicable to many situations where analytes must be detected in complex samples, with the main limiting factor being the availability of suitable DNA aptamers.
American Psychological Association (APA)
Smiley, Sally& DeRosa, Maria C.& Blais, Burton. 2013. Immobilization of DNA Aptamers on Polyester Cloth for Antigen Detection by Dot Blot Immunoenzymatic Assay (Aptablot). Journal of Nucleic Acids،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-509588
Modern Language Association (MLA)
Smiley, Sally…[et al.]. Immobilization of DNA Aptamers on Polyester Cloth for Antigen Detection by Dot Blot Immunoenzymatic Assay (Aptablot). Journal of Nucleic Acids No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-509588
American Medical Association (AMA)
Smiley, Sally& DeRosa, Maria C.& Blais, Burton. Immobilization of DNA Aptamers on Polyester Cloth for Antigen Detection by Dot Blot Immunoenzymatic Assay (Aptablot). Journal of Nucleic Acids. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-509588
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-509588