Human Hematopoietic Stem Cells Can Survive In Vitro for Several Months
Joint Authors
Hiroyama, Takashi
Nagasawa, Toshiro
Miharada, Kenichi
Chiba, Shigeru
Ninomiya, Haruhiko
Sudo, Kazuhiro
Ishigaki, Taro
Nakamura, Yukio
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-02-08
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
We previously reported that long-lasting in vitro hematopoiesis could be achieved using the cells differentiated from primate embryonic stem (ES) cells.
Thus, we speculated that hematopoietic stem cells differentiated from ES cells could sustain long-lasting in vitro hematopoiesis.
To test this hypothesis, we investigated whether human hematopoietic stem cells could similarly sustain long-lasting in vitro hematopoiesis in the same culture system.
Although the results varied between experiments, presumably due to differences in the quality of each hematopoietic stem cell sample, long-lasting in vitro hematopoiesis was observed to last up to nine months.
Furthermore, an in vivo analysis in which cultured cells were transplanted into immunodeficient mice indicated that even after several months of culture, hematopoietic stem cells were still present in the cultured cells.
To the best of our knowledge, this is the first report to show that human hematopoietic stem cells can survive in vitro for several months.
American Psychological Association (APA)
Ishigaki, Taro& Sudo, Kazuhiro& Hiroyama, Takashi& Miharada, Kenichi& Ninomiya, Haruhiko& Chiba, Shigeru…[et al.]. 2009. Human Hematopoietic Stem Cells Can Survive In Vitro for Several Months. Advances in Hematology،Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-509596
Modern Language Association (MLA)
Ishigaki, Taro…[et al.]. Human Hematopoietic Stem Cells Can Survive In Vitro for Several Months. Advances in Hematology No. 2009 (2009), pp.1-7.
https://search.emarefa.net/detail/BIM-509596
American Medical Association (AMA)
Ishigaki, Taro& Sudo, Kazuhiro& Hiroyama, Takashi& Miharada, Kenichi& Ninomiya, Haruhiko& Chiba, Shigeru…[et al.]. Human Hematopoietic Stem Cells Can Survive In Vitro for Several Months. Advances in Hematology. 2009. Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-509596
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-509596