Acetylshikonin, a Novel AChE Inhibitor, Inhibits Apoptosis via Upregulation of Heme Oxygenase-1 Expression in SH-SY5Y Cells
Joint Authors
Pan, Wen-Liang
Wang, Yan
Ng, Tzi-Bun
Law, Wai-Kit
Miu-Yee Waye, Mary
Chi-Cheong Wan, David
Tsz-Ming Ip, Denis
Liang, Wei-Cheng
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-05
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Acetylcholinesterase inhibitors are prominent alternative in current clinical treatment for AD patients.
Therefore, there is a continued need to search for novel AChEIs with good clinical efficacy and less side effects.
By using our in-house natural product database and AutoDock Vina as a tool in docking study, we have identified twelve phytochemicals (emodin, aloe-emodin, chrysophanol, and rhein in Rhei Radix Et Rhizoma; xanthotoxin, phellopterin, alloisoimperatorin, and imperatorin in Angelicae dahuricae Radix; shikonin, acetylshikonin, isovalerylshikonin, and β,β-dimethylacrylshikonin in Arnebiae Radix) as candidates of AChEIs that were not previously reported in the literature.
In addition to AChEI activity, a series of cell-based experiments were conducted for the investigation of their neuroprotective activities.
We found that acetylshikonin and its derivatives prevented apoptotic cell death induced by hydrogen peroxide in human and rat neuronal SH-SY5Y and PC12 cells at 10 μM.
We showed that acetylshikonin exhibited the most potent antiapoptosis activity through the inhibition of the generation of reactive oxygen species as well as protection of the loss of mitochondria membrane potential.
Furthermore, we identified for the first time that the upregulation of heme oxygenase 1 by acetylshikonin is a key step mediating its antiapoptotic activity from oxidative stress in SH-SY5Y cells.
American Psychological Association (APA)
Wang, Yan& Pan, Wen-Liang& Liang, Wei-Cheng& Law, Wai-Kit& Tsz-Ming Ip, Denis& Ng, Tzi-Bun…[et al.]. 2013. Acetylshikonin, a Novel AChE Inhibitor, Inhibits Apoptosis via Upregulation of Heme Oxygenase-1 Expression in SH-SY5Y Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-509657
Modern Language Association (MLA)
Wang, Yan…[et al.]. Acetylshikonin, a Novel AChE Inhibitor, Inhibits Apoptosis via Upregulation of Heme Oxygenase-1 Expression in SH-SY5Y Cells. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-509657
American Medical Association (AMA)
Wang, Yan& Pan, Wen-Liang& Liang, Wei-Cheng& Law, Wai-Kit& Tsz-Ming Ip, Denis& Ng, Tzi-Bun…[et al.]. Acetylshikonin, a Novel AChE Inhibitor, Inhibits Apoptosis via Upregulation of Heme Oxygenase-1 Expression in SH-SY5Y Cells. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-509657
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-509657