The Effect of an Aqueous Extract of Teucrium polium on Glutathione Homeostasis In Vitro : A Possible Mechanism of Its Hepatoprotectant Action
Joint Authors
Bomzon, Arieh
Shtukmaster, Stella
Ljubuncic, Predrag
Source
Advances in Pharmacological Sciences
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-03-23
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
Teucrium polium is used in Arab traditional medicine to treat liver diseases.
Glutathione is an important intracellular antioxidant, and intrahepatic glutathione levels are depleted in liver diseases.
Hypothesis and Aim.
This investigation tested the hypothesis that aqueous extracts of T.
polium maintains intracellular glutathione levels by augmenting glutathione peroxidase and glutathione reductase activity in cultured hepatocytes.
Methods.
The effects of increasing concentrations (0.01–1 mg/mL) of aqueous extract of T.
polium were assessed in cultured HepG2 cells following 24 hours incubation on (1) cellular integrity using (a) the Trypan blue exclusion assay, (b) the [di-methylthiazol-2yl]-2,5-diphenyl-tetrazoliumbromide (MTT) assay, and (c) the lactate dehydrogenase (LDH) assay; (2) glutathione redox state; and (3) glutathione peroxidase and glutathione reductase activities using a repeated measures experimental design.
Results.
At concentrations of 0.375 mg/mL and 0.5 mg/mL, the extract increased the intracellular levels of total and reduced glutathione and had no effect on the intracellular amounts of oxidized glutathione.
The extract had no effect on glutathione peroxidase and glutathione reductase activities.
Conclusion.
These data indicate that the mechanism of the hepatoprotective action of aqueous extracts of T.
polium may be, in part, due to augmenting intracellular glutathione levels.
American Psychological Association (APA)
Shtukmaster, Stella& Ljubuncic, Predrag& Bomzon, Arieh. 2010. The Effect of an Aqueous Extract of Teucrium polium on Glutathione Homeostasis In Vitro : A Possible Mechanism of Its Hepatoprotectant Action. Advances in Pharmacological Sciences،Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-509747
Modern Language Association (MLA)
Shtukmaster, Stella…[et al.]. The Effect of an Aqueous Extract of Teucrium polium on Glutathione Homeostasis In Vitro : A Possible Mechanism of Its Hepatoprotectant Action. Advances in Pharmacological Sciences No. 2010 (2010), pp.1-7.
https://search.emarefa.net/detail/BIM-509747
American Medical Association (AMA)
Shtukmaster, Stella& Ljubuncic, Predrag& Bomzon, Arieh. The Effect of an Aqueous Extract of Teucrium polium on Glutathione Homeostasis In Vitro : A Possible Mechanism of Its Hepatoprotectant Action. Advances in Pharmacological Sciences. 2010. Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-509747
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-509747