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Artificial Extracellular Matrices with Oversulfated Glycosaminoglycan Derivatives Promote the Differentiation of Osteoblast-Precursor Cells and Premature Osteoblasts
Joint Authors
Becher, Jana
Hintze, Vera
Vogel, Sarah
Preissler, Carolin
Hempel, Ute
Dieter, Peter
Hofbauer, Lorenz C.
Möller, Stephanie
Rauner, Martina
Schnabelrauch, Matthias
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-27
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Sulfated glycosaminoglycans (GAG) are components of the bone marrow stem cell niche and to a minor extent of mature bone tissue with important functions in regulating stem cell lineage commitment and differentiation.
We anticipated that artificial extracellular matrices (aECM) composed of collagen I and synthetically oversulfated GAG derivatives affect preferentially the differentiation of osteoblast-precursor cells and early osteoblasts.
A set of gradually sulfated chondroitin sulfate and hyaluronan derivatives was used for the preparation of aECM.
All these matrices were analysed with human bone marrow stromal cells to identify the most potent aECM and to determine the influence of the degree and position of sulfate groups and the kind of disaccharide units on the osteogenic differentiation.
Oversulfated GAG derivatives with a sulfate group at the C-6 position of the N-acetylglycosamine revealed the most pronounced proosteogenic effect as determined by tissue nonspecific alkaline phosphatase activity and calcium deposition.
A subset of the aECM was further analysed with different primary osteoblasts and cell lines reflecting different maturation stages to test whether the effect of sulfated GAG derivatives depends on the maturation status of the cells.
It was shown that the proosteogenic effect of aECM was most prominent in early osteoblasts.
American Psychological Association (APA)
Hempel, Ute& Preissler, Carolin& Vogel, Sarah& Möller, Stephanie& Hintze, Vera& Becher, Jana…[et al.]. 2014. Artificial Extracellular Matrices with Oversulfated Glycosaminoglycan Derivatives Promote the Differentiation of Osteoblast-Precursor Cells and Premature Osteoblasts. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-509750
Modern Language Association (MLA)
Hempel, Ute…[et al.]. Artificial Extracellular Matrices with Oversulfated Glycosaminoglycan Derivatives Promote the Differentiation of Osteoblast-Precursor Cells and Premature Osteoblasts. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-509750
American Medical Association (AMA)
Hempel, Ute& Preissler, Carolin& Vogel, Sarah& Möller, Stephanie& Hintze, Vera& Becher, Jana…[et al.]. Artificial Extracellular Matrices with Oversulfated Glycosaminoglycan Derivatives Promote the Differentiation of Osteoblast-Precursor Cells and Premature Osteoblasts. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-509750
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-509750