Establishment of Methylation-Specific PCR for the Mouse p53 Gene
Joint Authors
Ootsuyama, Akira
Okazaki, Ryuji
Norimura, Toshiyuki
Yoshida, Yasuhiro
Source
Molecular Biology International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-12
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Methylation-specific PCR (MSP) of the mouse p53 gene has not yet been reported.
We searched the CpG islands, sequenced the bisulfited DNA, and designed PCR primers for methylation and unmethylation sites.
DNA from a young mouse produced a strong PCR product with the unmethylated primer and a weaker band with the methylated primer.
DNA from an old mouse produced bands of similar intensities with both primers.
In radiation-induced tumors, DNA from an old mouse yielded similar bands with both types of primers.
We suggest that MSP is a valuable technique for the epigenetic study of the mouse p53 gene.
American Psychological Association (APA)
Okazaki, Ryuji& Ootsuyama, Akira& Yoshida, Yasuhiro& Norimura, Toshiyuki. 2011. Establishment of Methylation-Specific PCR for the Mouse p53 Gene. Molecular Biology International،Vol. 2011, no. 2011, pp.1-4.
https://search.emarefa.net/detail/BIM-509758
Modern Language Association (MLA)
Okazaki, Ryuji…[et al.]. Establishment of Methylation-Specific PCR for the Mouse p53 Gene. Molecular Biology International No. 2011 (2011), pp.1-4.
https://search.emarefa.net/detail/BIM-509758
American Medical Association (AMA)
Okazaki, Ryuji& Ootsuyama, Akira& Yoshida, Yasuhiro& Norimura, Toshiyuki. Establishment of Methylation-Specific PCR for the Mouse p53 Gene. Molecular Biology International. 2011. Vol. 2011, no. 2011, pp.1-4.
https://search.emarefa.net/detail/BIM-509758
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-509758
