Analysis of the Bacterial Diversity Associated with the Roots of Maize (Zea mays L.) through Culture-Dependent and Culture-Independent Methods
Joint Authors
Ibáñez, Fernando
Martínez-Romero, Esperanza
Rosenblueth, Mónica
Pereira, Paola
Etcheverry, Miriam
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-03
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Earth Science , Water and Environment
Abstract EN
The present study investigated bacterial diversity associated with the roots of maize through the use of culture-dependent and culture-independent methods.
Bacterial 16S–23S rDNA internal transcribed spacer sequences (ITS) primers were used to amplify sequences obtained directly from the root matrix by Percoll gradient separation.
This assay showed that γ-Proteobacteria within Enterobacter, Erwinia, Klebsiella, Pseudomonas, and Stenotrophomonas genera were predominant groups.
The culturable component of the bacterial community was also assessed, revealing that the predominant group was Firmicutes, mainly of Bacillus genus, while Achromobacter, Lysinibacillus, and Paenibacillus genera were rarely found in association with the roots.
Only two genera within γ-Proteobacteria, Enterobacter and Pseudomonas, were found in the culture collection.
Differences in richness and diversity between the rhizospheric and endophytic bacterial communities were also evidenced.
The spectrum of bacteria naturally associated with maize roots is wide and the magnitude of such diversity will depend on the methods chosen for analysis.
The knowledge of this spectrum will facilitate the search of microorganisms capable of exerting antagonism to diverse pathogens or detecting plant growth enhancers.
American Psychological Association (APA)
Pereira, Paola& Ibáñez, Fernando& Rosenblueth, Mónica& Etcheverry, Miriam& Martínez-Romero, Esperanza. 2011. Analysis of the Bacterial Diversity Associated with the Roots of Maize (Zea mays L.) through Culture-Dependent and Culture-Independent Methods. ISRN Ecology،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-509771
Modern Language Association (MLA)
Pereira, Paola…[et al.]. Analysis of the Bacterial Diversity Associated with the Roots of Maize (Zea mays L.) through Culture-Dependent and Culture-Independent Methods. ISRN Ecology No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-509771
American Medical Association (AMA)
Pereira, Paola& Ibáñez, Fernando& Rosenblueth, Mónica& Etcheverry, Miriam& Martínez-Romero, Esperanza. Analysis of the Bacterial Diversity Associated with the Roots of Maize (Zea mays L.) through Culture-Dependent and Culture-Independent Methods. ISRN Ecology. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-509771
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-509771