Kinetic Characterisation of Phosphofructokinase Purified from Setaria cervi : A Bovine Filarial Parasite
Author
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-15
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Phosphofructokinase (PFK), a regulatory enzyme in glycolytic pathway, has been purified to electrophoretic homogeneity from adult female Setaria cervi and partially characterized.
For this enzyme, the Lineweaver-Burk's double reciprocal plots of initial rates and D-fructose-6-phosphate (F-6-P) or Mg-ATP concentrations for varying values of cosubstrate concentration gave intersecting lines indicating that Km values for F-6-P (1.05 mM) and ATP (3 μM) were independent of each other.
S.
cervi PFK, when assayed at inhibitory concentration of ATP (>0.1 mM), exhibited sigmoidal behavior towards binding with F-6-P with a Hill coefficient (n) value equal to 1.8 and 1.7 at 1.0 and 0.33 mM ATP, respectively.
D-fructose-1,6-diphosphate (FDP) competitively inhibited the filarial enzyme: Ki and Hill coefficient values being 0.18 μM and 2.0, respectively.
Phosphoenolpyruvate (PEP) also inhibited the enzyme competitively with the Ki value equal to 0.8 mM.
The Hill coefficient values (>1.5) for F-6-P (at inhibitory concentration of ATP) and FDP suggested its positive cooperative kinetics towards F-6-P and FDP, showing presence of more than one binding sites for these molecules in enzyme protein and allosteric nature of the filarial enzyme.
The product inhibition studies gave us the only compatible mechanism of random addition process with a probable orientation of substrates and products on the enzyme surface.
American Psychological Association (APA)
Sharma, Bechan. 2011. Kinetic Characterisation of Phosphofructokinase Purified from Setaria cervi : A Bovine Filarial Parasite. Enzyme Research،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-509836
Modern Language Association (MLA)
Sharma, Bechan. Kinetic Characterisation of Phosphofructokinase Purified from Setaria cervi : A Bovine Filarial Parasite. Enzyme Research No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-509836
American Medical Association (AMA)
Sharma, Bechan. Kinetic Characterisation of Phosphofructokinase Purified from Setaria cervi : A Bovine Filarial Parasite. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-509836
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-509836