Spectrofluorimetric Determination of Human Serum Albumin Using Terbium-Danofloxacin Probe

Abstract EN

A spectrofluorimetric method is proposed for the determination of human serum albumin (HSA) and bovine serum albumin (BSA) using terbium-danofloxacin (Tb3+-Dano) as a fluorescent probe.

These proteins remarkably enhance the fluorescence intensity of the Tb3+-Dano complex at 545 nm, and the enhanced fluorescence intensity of Tb3+-Dano is proportional to the concentration of proteins (HSA and BSA).

Optimum conditions for the determination of HSA were investigated and found that the maximum response was observed at: pH=7.8, [Tb3+] =8.5×10-5 mol L−1, [Dano] =1.5×10-4 mol L−1.

The calibration graphs for standard solutions of BSA, HSA, and plasma samples of HSA were linear in the range of 0.2×10−6−1.3×10−6 mol L−1, 0.2×10−6-1.4×10−6 mol L−1, and 0.2×10-6−1×10−6 mol L−1, respectively.

The detection limits (S/N = 3) for BSA, HSA, and plasma sample of HSA were 8.7×10-8 mol L−1, 6.2×10−8 mol L−1, and 8.1×10−8 mol L−1, respectively.

The applicability of the method was checked using a number of real biological plasma samples and was compared with the UV spectrometric reference method.

The results was showed that the method could be regarded as a simple, practical, and sensitive alternative method for determination of albumin in biological samples.