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Upregulation of Pluripotency Markers in Adipose Tissue-Derived Stem Cells by miR-302 and Leukemia Inhibitory Factor
Joint Authors
Javeri, Arash
Rohban, Sara
Mowla, Seyed Javad
Taha, Masoumeh Fakhr
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-23
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The expression pattern of pluripotency markers in adipose tissue-derived stem cells (ADSCs) is a subject of controversy.
Moreover, there is no data about the signaling molecules that regulate these markers in ADSCs.
In the present study, we studied the roles of leukemia inhibitory factor (LIF) and miR-302 in this regard.
Freshly isolated mouse ADSCs expressed hematopoietic, mesenchymal, and pluripotency markers.
One day after plating, ADSCs expressed OCT4 and Sox2 proteins.
After three passages, the expression of hematopoietic and pluripotency markers decreased, while the expression of mesenchymal stem cell markers exhibited a striking rise.
Both supplementation of culture media with LIF and transfection of the ADSCs with miR-302 family upregulated the expression levels of OCT4, Nanog, and Sox2 mRNAs.
These findings showed that mouse adipose tissue contains a population of cells with molecular resemblance to embryonic stem cells, and LIF and miR-302 family positively affect the expression of pluripotency markers.
American Psychological Association (APA)
Taha, Masoumeh Fakhr& Javeri, Arash& Rohban, Sara& Mowla, Seyed Javad. 2014. Upregulation of Pluripotency Markers in Adipose Tissue-Derived Stem Cells by miR-302 and Leukemia Inhibitory Factor. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-510016
Modern Language Association (MLA)
Taha, Masoumeh Fakhr…[et al.]. Upregulation of Pluripotency Markers in Adipose Tissue-Derived Stem Cells by miR-302 and Leukemia Inhibitory Factor. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-510016
American Medical Association (AMA)
Taha, Masoumeh Fakhr& Javeri, Arash& Rohban, Sara& Mowla, Seyed Javad. Upregulation of Pluripotency Markers in Adipose Tissue-Derived Stem Cells by miR-302 and Leukemia Inhibitory Factor. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-510016
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-510016