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Mass Spectrometric Analysis of Multiple Pertussis Toxins and Toxoids
Joint Authors
Williamson, Yulanda M.
Pirkle, James L.
Woolfitt, Adrian R.
Sampson, Jacquelyn S.
Carlone, George
Ades, Edwin W.
Tondella, Maria L.
Moura, Hercules
Rees, Jon
Schieltz, David
Barr, John R.
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-05-23
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Bordetella pertussis (Bp) is the causative agent of pertussis, a vaccine preventable disease occurring primarily in children.
In recent years, there has been increased reporting of pertussis.
Current pertussis vaccines are acellular and consist of Bp proteins including the major virulence factor pertussis toxin (Ptx), a 5-subunit exotoxin.
Variation in Ptx subunit amino acid (AA) sequence could possibly affect the immune response.
A blind comparative mass spectrometric (MS) analysis of commercially available Ptx as well as the chemically modified toxoid (Ptxd) from licensed vaccines was performed to assess peptide sequence and AA coverage variability as well as relative amounts of Ptx subunits.
Qualitatively, there are similarities among the various sources based on AA percent coverages and MS/MS fragmentation profiles.
Additionally, based on a label-free mass spectrometry-based quantification method there is differential relative abundance of the subunits among the sources.
American Psychological Association (APA)
Williamson, Yulanda M.& Moura, Hercules& Schieltz, David& Rees, Jon& Woolfitt, Adrian R.& Pirkle, James L.…[et al.]. 2010. Mass Spectrometric Analysis of Multiple Pertussis Toxins and Toxoids. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-510110
Modern Language Association (MLA)
Williamson, Yulanda M.…[et al.]. Mass Spectrometric Analysis of Multiple Pertussis Toxins and Toxoids. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-9.
https://search.emarefa.net/detail/BIM-510110
American Medical Association (AMA)
Williamson, Yulanda M.& Moura, Hercules& Schieltz, David& Rees, Jon& Woolfitt, Adrian R.& Pirkle, James L.…[et al.]. Mass Spectrometric Analysis of Multiple Pertussis Toxins and Toxoids. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-510110
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-510110