Affinity and Matrix Effects in Measuring Fish Plasma Vitellogenin Using Immunosorbent Assays : Considerations for Aquatic Toxicologists
Joint Authors
Bartell, Stephen E.
Schoenfuss, Heiko L.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-18
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Enzyme-linked immunosorbent assays (ELISAs) are important tools in aquatic toxicology and have become crucial in assessing exposure concentrations in the aquatic environment and acute physiological responses in exposed organisms.
These assays utilize the inherent properties of antibodies to recognize and selectively bind a target molecule, while largely ignoring other molecules to provide semiquantitative values.
A variety of methodologies to measure plasma vitellogenin using ELISAs have generated widely divergent data.
Limitations of the ELISA method are known in the wider immunology field, though aquatic toxicologists may be less familiar with these limitations.
We evaluated several mechanisms contributing to the divergent vitellogenin data in the literature.
Antibody affinities and the matrix in which standard curves are constructed are possible error generators.
These errors can be amplified by large sample dilutions necessary to fall within the standard curve.
It is important for the aquatic toxicology research community to realize the limitations and understand the pitfalls of absolute plasma vitellogenin data in their studies.
American Psychological Association (APA)
Bartell, Stephen E.& Schoenfuss, Heiko L.. 2012. Affinity and Matrix Effects in Measuring Fish Plasma Vitellogenin Using Immunosorbent Assays : Considerations for Aquatic Toxicologists. ISRN Toxicology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-510147
Modern Language Association (MLA)
Bartell, Stephen E.& Schoenfuss, Heiko L.. Affinity and Matrix Effects in Measuring Fish Plasma Vitellogenin Using Immunosorbent Assays : Considerations for Aquatic Toxicologists. ISRN Toxicology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-510147
American Medical Association (AMA)
Bartell, Stephen E.& Schoenfuss, Heiko L.. Affinity and Matrix Effects in Measuring Fish Plasma Vitellogenin Using Immunosorbent Assays : Considerations for Aquatic Toxicologists. ISRN Toxicology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-510147
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-510147