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Muscle-Type Specific Autophosphorylation of CaMKII Isoforms after Paced Contractions
Joint Authors
van Overbeek, Daniëlle
de Haan, Arnold
Gevers, Wouter
Flück, Martin
Eilers, Wouter
Hilbers, Peter A.
van Riel, Natal
Jaspers, Richard T.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-20, 20 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-26
Country of Publication
Egypt
No. of Pages
20
Main Subjects
Abstract EN
We explored to what extent isoforms of the regulator of excitation-contraction and excitation-transcription coupling, calcium/calmodulin protein kinase II (CaMKII) contribute to the specificity of myocellular calcium sensing between muscle types and whether concentration transients in its autophosphorylation can be simulated.
CaMKII autophosphorylation at Thr287 was assessed in three muscle compartments of the rat after slow or fast motor unit-type stimulation and was compared against a computational model (CaMuZclE) coupling myocellular calcium dynamics with CaMKII Thr287 phosphorylation.
Qualitative differences existed between fast- (gastrocnemius medialis) and slow-type muscle (soleus) for the expression pattern of CaMKII isoforms.
Phospho-Thr287 content of δA CaMKII, associated with nuclear functions, demonstrated a transient and compartment-specific increase after excitation, which contrasted to the delayed autophosphorylation of the sarcoplasmic reticulum-associated βM CaMKII.
In soleus muscle, excitation-induced δA CaMKII autophosphorylation demonstrated frequency dependence (P = 0.02).
In the glycolytic compartment of gastrocnemius medialis, CaMKII autophosphorylation after excitation was blunted.
In silico assessment emphasized the importance of mitochondrial calcium buffer capacity for excitation-induced CaMKII autophosphorylation but did not predict its isoform specificity.
The findings expose that CaMKII autophosphorylation with paced contractions is regulated in an isoform and muscle type-specific fashion and highlight properties emerging for phenotype-specific regulation of CaMKII.
American Psychological Association (APA)
Eilers, Wouter& Gevers, Wouter& van Overbeek, Daniëlle& de Haan, Arnold& Jaspers, Richard T.& Hilbers, Peter A.…[et al.]. 2014. Muscle-Type Specific Autophosphorylation of CaMKII Isoforms after Paced Contractions. BioMed Research International،Vol. 2014, no. 2014, pp.1-20.
https://search.emarefa.net/detail/BIM-510233
Modern Language Association (MLA)
Eilers, Wouter…[et al.]. Muscle-Type Specific Autophosphorylation of CaMKII Isoforms after Paced Contractions. BioMed Research International No. 2014 (2014), pp.1-20.
https://search.emarefa.net/detail/BIM-510233
American Medical Association (AMA)
Eilers, Wouter& Gevers, Wouter& van Overbeek, Daniëlle& de Haan, Arnold& Jaspers, Richard T.& Hilbers, Peter A.…[et al.]. Muscle-Type Specific Autophosphorylation of CaMKII Isoforms after Paced Contractions. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-20.
https://search.emarefa.net/detail/BIM-510233
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-510233