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Nona-Arginine Facilitates Delivery of Quantum Dots into Cells via Multiple Pathways
Joint Authors
Wang, Tien-Chun
Shannon, Katie B.
Liu, Betty Revon
Chiang, Huey-Jenn
Xu, Yi
Winiarz, Jeffrey G.
Huang, Yue-wern
Lee, Han-Jung
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-10-27
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Semiconductor quantum dots (QDs) have recently been used to deliver and monitor biomolecules, such as drugs and proteins.
However, QDs alone have a low efficiency of transport across the plasma membrane.
In order to increase the efficiency, we used synthetic nona-arginine (SR9), a cell-penetrating peptide, to facilitate uptake.
We found that SR9 increased the cellular uptake of QDs in a noncovalent binding manner between QDs and SR9.
Further, we investigated mechanisms of QD/SR9 cellular internalization.
Low temperature and metabolic inhibitors markedly inhibited the uptake of QD/SR9, indicating that internalization is an energy-dependent process.
Results from both the pathway inhibitors and the RNA interference (RNAi) technique suggest that cellular uptake of QD/SR9 is predominantly a lipid raft-dependent process mediated by macropinocytosis.
However, involvement of clathrin and caveolin-1 proteins in transducing QD/SR9 across the membrane cannot be completely ruled out.
American Psychological Association (APA)
Xu, Yi& Liu, Betty Revon& Lee, Han-Jung& Shannon, Katie B.& Winiarz, Jeffrey G.& Wang, Tien-Chun…[et al.]. 2010. Nona-Arginine Facilitates Delivery of Quantum Dots into Cells via Multiple Pathways. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-510580
Modern Language Association (MLA)
Xu, Yi…[et al.]. Nona-Arginine Facilitates Delivery of Quantum Dots into Cells via Multiple Pathways. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-11.
https://search.emarefa.net/detail/BIM-510580
American Medical Association (AMA)
Xu, Yi& Liu, Betty Revon& Lee, Han-Jung& Shannon, Katie B.& Winiarz, Jeffrey G.& Wang, Tien-Chun…[et al.]. Nona-Arginine Facilitates Delivery of Quantum Dots into Cells via Multiple Pathways. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-510580
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-510580