Murine Aortic Smooth Muscle Cells Acquire, Though Fail to Present Exogenous Protein Antigens on Major Histocompatibility Complex Class II Molecules
Joint Authors
Brewer, James M.
Maddaluno, Marcella
Ialenti, Armando
Grassia, Gianluca
Maffia, Pasquale
Butcher, John P.
MacRitchie, Neil
Garside, Paul
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-20
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
In the present study aortic murine smooth muscle cell (SMC) antigen presentation capacity was evaluated using the Eα-GFP/Y-Ae system to visualize antigen uptake through a GFP tag and tracking of Eα peptide/MHCII presentation using the Y-Ae Ab.
Stimulation with IFN-γ (100 ng/mL) for 72 h caused a significant (P<0.01) increase in the percentage of MHC class II positive SMCs, compared with unstimulated cells.
Treatment with Eα-GFP (100 μg/mL) for 48 h induced a significant (P<0.05) increase in the percentage of GFP positive SMCs while it did not affect the percentage of Y-Ae positive cells, being indicative of antigen uptake without its presentation in the context of MHC class II.
After IFN-γ-stimulation, ovalbumin- (OVA, 1 mg/mL) or OVA323–339 peptide-(0.5 μg/mL) treated SMCs failed to induce OT-II CD4+ T cell activation/proliferation; this was also accompanied by a lack of expression of key costimulatory molecules (OX40L, CD40, CD70, and CD86) on SMCs.
Finally, OVA-treated SMCs failed to induce DO11.10-GFP hybridoma activation, a process independent of costimulation.
Our results demonstrate that while murine primary aortic SMCs express MHC class II and can acquire exogenous antigens, they fail to activate T cells through a failure in antigen presentation and a lack of costimulatory molecule expression.
American Psychological Association (APA)
Maddaluno, Marcella& MacRitchie, Neil& Grassia, Gianluca& Ialenti, Armando& Butcher, John P.& Garside, Paul…[et al.]. 2014. Murine Aortic Smooth Muscle Cells Acquire, Though Fail to Present Exogenous Protein Antigens on Major Histocompatibility Complex Class II Molecules. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-510690
Modern Language Association (MLA)
Maddaluno, Marcella…[et al.]. Murine Aortic Smooth Muscle Cells Acquire, Though Fail to Present Exogenous Protein Antigens on Major Histocompatibility Complex Class II Molecules. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-510690
American Medical Association (AMA)
Maddaluno, Marcella& MacRitchie, Neil& Grassia, Gianluca& Ialenti, Armando& Butcher, John P.& Garside, Paul…[et al.]. Murine Aortic Smooth Muscle Cells Acquire, Though Fail to Present Exogenous Protein Antigens on Major Histocompatibility Complex Class II Molecules. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-510690
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-510690