In Silico Characterization of Pectate Lyase Protein Sequences from Different Source Organisms
Joint Authors
Dubey, Amit Kumar
Yadav, Sangeeta
Sarangi, Bijaya Ketan
Kumar, Manish
Yadav, Dinesh
Singh, Vinay Kumar
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-09-19
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
A total of 121 protein sequences of pectate lyases were subjected to homology search, multiple sequence alignment, phylogenetic tree construction, and motif analysis.
The phylogenetic tree constructed revealed different clusters based on different source organisms representing bacterial, fungal, plant, and nematode pectate lyases.
The multiple accessions of bacterial, fungal, nematode, and plant pectate lyase protein sequences were placed closely revealing a sequence level similarity.
The multiple sequence alignment of these pectate lyase protein sequences from different source organisms showed conserved regions at different stretches with maximum homology from amino acid residues 439–467, 715–816, and 829–910 which could be used for designing degenerate primers or probes specific for pectate lyases.
The motif analysis revealed a conserved Pec_Lyase_C domain uniformly observed in all pectate lyases irrespective of variable sources suggesting its possible role in structural and enzymatic functions.
American Psychological Association (APA)
Dubey, Amit Kumar& Yadav, Sangeeta& Kumar, Manish& Singh, Vinay Kumar& Sarangi, Bijaya Ketan& Yadav, Dinesh. 2010. In Silico Characterization of Pectate Lyase Protein Sequences from Different Source Organisms. Enzyme Research،Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-510712
Modern Language Association (MLA)
Dubey, Amit Kumar…[et al.]. In Silico Characterization of Pectate Lyase Protein Sequences from Different Source Organisms. Enzyme Research No. 2010 (2010), pp.1-11.
https://search.emarefa.net/detail/BIM-510712
American Medical Association (AMA)
Dubey, Amit Kumar& Yadav, Sangeeta& Kumar, Manish& Singh, Vinay Kumar& Sarangi, Bijaya Ketan& Yadav, Dinesh. In Silico Characterization of Pectate Lyase Protein Sequences from Different Source Organisms. Enzyme Research. 2010. Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-510712
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-510712