Biochemical and Functional Characterization of Parawixia bistriata Spider Venom with Potential Proteolytic and Larvicidal Activities
Joint Authors
de Almeida e Silva, Alexandre
da Silva, Saulo Luís
Zuliani, Juliana P.
Kayano, Anderson M.
Trindade, Frances
Gimenez, Gizeli S.
Stábeli, Rodrigo G.
Marcussi, Silvana
Coutinho-Neto, Antonio
Calderon, Leonardo A.
Soares, Andreimar Martins
Simões-Silva, Rodrigo
Fernandes, Carla F. C.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-07
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Toxins purified from the venom of spiders have high potential to be studied pharmacologically and biochemically.
These biomolecules may have biotechnological and therapeutic applications.
This study aimed to evaluate the protein content of Parawixia bistriata venom and functionally characterize its proteins that have potential for biotechnological applications.
The crude venom showed no phospholipase, hemorrhagic, or anti-Leishmania activities attesting to low genotoxicity and discrete antifungal activity for C.
albicans.
However the following activities were observed: anticoagulation, edema, myotoxicity and proteolysis on casein, azo-collagen, and fibrinogen.
The chromatographic and electrophoretic profiles of the proteins revealed a predominance of acidic, neutral, and polar proteins, highlighting the presence of proteins with high molecular masses.
Five fractions were collected using cation exchange chromatography, with the P4 fraction standing out as that of the highest purity.
All fractions showed proteolytic activity.
The crude venom and fractions P1, P2, and P3 showed larvicidal effects on A.
aegypti.
Fraction P4 showed the presence of a possible metalloprotease (60 kDa) that has high proteolytic activity on azo-collagen and was inhibited by EDTA.
The results presented in this study demonstrate the presence of proteins in the venom of P.
bistriata with potential for biotechnological applications.
American Psychological Association (APA)
Gimenez, Gizeli S.& Coutinho-Neto, Antonio& Kayano, Anderson M.& Simões-Silva, Rodrigo& Trindade, Frances& de Almeida e Silva, Alexandre…[et al.]. 2014. Biochemical and Functional Characterization of Parawixia bistriata Spider Venom with Potential Proteolytic and Larvicidal Activities. BioMed Research International،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-510754
Modern Language Association (MLA)
Gimenez, Gizeli S.…[et al.]. Biochemical and Functional Characterization of Parawixia bistriata Spider Venom with Potential Proteolytic and Larvicidal Activities. BioMed Research International No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-510754
American Medical Association (AMA)
Gimenez, Gizeli S.& Coutinho-Neto, Antonio& Kayano, Anderson M.& Simões-Silva, Rodrigo& Trindade, Frances& de Almeida e Silva, Alexandre…[et al.]. Biochemical and Functional Characterization of Parawixia bistriata Spider Venom with Potential Proteolytic and Larvicidal Activities. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-510754
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-510754