PCR-Based Detection and Genotyping of Helicobacter pylori in Endoscopic Biopsy Samples from Brazilian Patients
Joint Authors
Zeitune, José Murilo Robilota
Bonon, Sandra Helena Alves
Costa, Sandra Cecília Botelho
Menoni, Silvia M. Ferreira
Source
Gastroenterology Research and Practice
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-16
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Helicobacter pylori (H.
pylori) is considered the second most prevalent infection in man.
A precise diagnosis is important for treating patients with the indicative gastrointestinal symptoms.
The present study analyzes the effectiveness of a molecular biology method (PCR) comparing the results obtained with the histology and with the rapid urease tests.
PCR was used in the detection and genotyping of the H.
pylori urease-C gene and the patterns which were obtained from the patients studied.
141 biopsy samples from 131 patients were evaluated.
59 paraffin biopsies samples were positive for H.
pylori according to the histological examination.
Of those, 59/12 (20.3%) were amplified using PCR.
Of the 82 samples from the fresh biopsies, 64 were positive for H.
pylori according to the rapid urease test (78%); there was an agreement of 100% with PCR.
Sixty positive H.
pylori samples were genotyped (58 samples of fresh biopsies and 2 samples of paraffin biopsies) using two restriction enzymes.
The patterns observed were analyzed with the computational program BIO 1D; 11 patterns with the enzyme HhaI and 12 patterns with the enzyme MboI were found.
However, it was not possible to find a statistically significant correlation between the specific genotypes and digestive pathologies.
Accordingly, future research should be performed to confirm a statistically significant relationship between genotyping and gastrointestinal symptoms.
American Psychological Association (APA)
Menoni, Silvia M. Ferreira& Bonon, Sandra Helena Alves& Zeitune, José Murilo Robilota& Costa, Sandra Cecília Botelho. 2013. PCR-Based Detection and Genotyping of Helicobacter pylori in Endoscopic Biopsy Samples from Brazilian Patients. Gastroenterology Research and Practice،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-510802
Modern Language Association (MLA)
Menoni, Silvia M. Ferreira…[et al.]. PCR-Based Detection and Genotyping of Helicobacter pylori in Endoscopic Biopsy Samples from Brazilian Patients. Gastroenterology Research and Practice No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-510802
American Medical Association (AMA)
Menoni, Silvia M. Ferreira& Bonon, Sandra Helena Alves& Zeitune, José Murilo Robilota& Costa, Sandra Cecília Botelho. PCR-Based Detection and Genotyping of Helicobacter pylori in Endoscopic Biopsy Samples from Brazilian Patients. Gastroenterology Research and Practice. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-510802
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-510802