One Single Amino Acid for Estimation the Content of Total Free Amino Acids in Qingkailing Injection Using High-Performance Liquid Chromatography-Diode Array Detection
Joint Authors
Zhang, Zhixin
Gao, Xiaoyan
Zhang, Li
Su, Jiankun
Guo, Mingxing
He, Cheng
Peng, Long
Liu, Haiyu
Wang, Xue
Qin, Lingling
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-13
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Qingkailing injection (QKLI), a modern traditional Chinese medicine preparation, has been widely used in clinics due to its fast and significant efficacy in treatment of high fever.
The free amino acids (AAs) were considered to be the most abundant active ingredients indisputably.
So developing an accurate and simple determination method to measure the contents of total free AAs in QKLI is very crucial.
In current study, the accurate and simple method of using one single standard AA for simultaneous quantification of multiple AAs (One for M) in QKLI was developed.
Particularly, the calculation methods and the robustness of relative correction factors (RCFs) were investigated systematically.
No statistically significant difference between these two quantification methods of One for M and classic regression equation was found by the t-test (P = 95%, P>0.05).
The results showed that the precision (RSD < 4.88%), the robustness (RSD < 4.04%), and the average recoveries (94.11%–107.94%) of this newly proposed method all met the requirements for content determination.
This One for M method will provide a scientific reference for the quantitative determination of AAs in other traditional Chinese medicines and their preparations owing to its accuracy and simplicity.
American Psychological Association (APA)
Zhang, Li& Wang, Xue& Su, Jiankun& Liu, Haiyu& Zhang, Zhixin& Qin, Lingling…[et al.]. 2014. One Single Amino Acid for Estimation the Content of Total Free Amino Acids in Qingkailing Injection Using High-Performance Liquid Chromatography-Diode Array Detection. Journal of Analytical Methods in Chemistry،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-510807
Modern Language Association (MLA)
Zhang, Li…[et al.]. One Single Amino Acid for Estimation the Content of Total Free Amino Acids in Qingkailing Injection Using High-Performance Liquid Chromatography-Diode Array Detection. Journal of Analytical Methods in Chemistry No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-510807
American Medical Association (AMA)
Zhang, Li& Wang, Xue& Su, Jiankun& Liu, Haiyu& Zhang, Zhixin& Qin, Lingling…[et al.]. One Single Amino Acid for Estimation the Content of Total Free Amino Acids in Qingkailing Injection Using High-Performance Liquid Chromatography-Diode Array Detection. Journal of Analytical Methods in Chemistry. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-510807
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-510807