![](/images/graphics-bg.png)
Anti-EphA2 Antibodies with Distinct In Vitro Properties Have Equal In Vivo Efficacy in Pancreatic Cancer
Joint Authors
Santini, Claudia
Mori, Federica
Acali, Stefano
Vitelli, Alessandra
Pezzanera, Monica
Ciliberto, Gennaro
Ansuini, Helenia
Luzzago, Alessandra
Paradisi, Valentina
Lazzaro, Domenico
La Monica, Nicola
Meola, Annalisa
Nicosia, Alfredo
Gunes, Zeynep
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-01-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The EphA2 receptor tyrosine kinase is overexpressed in a variety of human epithelial cancers and is a determinant of malignant cellular behavior in pancreatic adenocarcinoma cells.
Moreover, it is expressed in tumor endothelium and its activation promotes angiogenesis.
To better clarify the therapeutic potential of monoclonal antibodies (mAbs) directed to the EphA2 receptor, we generated a large number of mAbs by differential screening of phage-Ab libraries by oligonucleotide microarray technology and implemented a strategy for the rapid identification of antibodies with the desired properties.
We selected two high-affinity and highly specific EphA2 monoclonal antibodies with different in vitro properties on the human pancreatic tumor cell line MiaPaCa2.
One is a potent EphA2-agonistic antibody, IgG25, that promotes receptor endocytosis and subsequent degradation, and the second is a ligand antagonist, IgG28, that blocks the binding to ephrin A1 and is cross-reactive with the mouse EphA2 receptor.
We measured the effect of antibody treatment on the growth of MiaPaCa2 cells orthotopically transplanted in nude mice.
Both IgG25 and IgG28 had strong antitumor and antimetastatic efficacy.
In vivo treatment with IgG25 determined the reduction of the EphA2 protein levels in the tumor and the phosphorylation of FAK on Tyr576 while administration of IgG28 caused a decrease in tumor vascularization as measured by immunohistochemical analysis of CD31 in tumor sections.
These data show that in a pancreatic cancer model comparable therapeutic efficacy is obtained either by promoting receptor degradation or by blocking receptor activation.
American Psychological Association (APA)
Ansuini, Helenia& Meola, Annalisa& Gunes, Zeynep& Paradisi, Valentina& Pezzanera, Monica& Acali, Stefano…[et al.]. 2010. Anti-EphA2 Antibodies with Distinct In Vitro Properties Have Equal In Vivo Efficacy in Pancreatic Cancer. Journal of Oncology،Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-510902
Modern Language Association (MLA)
Ansuini, Helenia…[et al.]. Anti-EphA2 Antibodies with Distinct In Vitro Properties Have Equal In Vivo Efficacy in Pancreatic Cancer. Journal of Oncology No. 2009 (2009), pp.1-10.
https://search.emarefa.net/detail/BIM-510902
American Medical Association (AMA)
Ansuini, Helenia& Meola, Annalisa& Gunes, Zeynep& Paradisi, Valentina& Pezzanera, Monica& Acali, Stefano…[et al.]. Anti-EphA2 Antibodies with Distinct In Vitro Properties Have Equal In Vivo Efficacy in Pancreatic Cancer. Journal of Oncology. 2010. Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-510902
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-510902