Novel miR390-Dependent Transacting siRNA Precursors in Plants Revealed by a PCR-Based Experimental Approach and Database Analysis
Joint Authors
Ozerova, L. V.
Milyutina, I. A.
Troitsky, A. V.
Morozov, Sergey Y.
Solovyev, A. G.
Krasnikova, M. S.
Bobrova, V. K.
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-10-13
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
TAS loci in plant genomes encode transacting small interfering RNAs (ta-siRNAs) that regulate expression of a number of genes.
The function of TAS3 precursor in Arabidopsis thaliana is controlled by two miR390 target sites flanking two ta-siARF sequences targeting mRNAs of ARF transcription factors.
Cleavage of the 3′-miR390-site initiates ta-siRNAs biogenesis.
Here we describe the new method for identification of plant ta-siRNA precursors based on PCR with oligodeoxyribonucleotide primers mimicking miR390.
The method was found to be efficient for dicotiledonous plants, cycads, and mosses.
Based on sequences of amplified loci and a database analysis, a novel type of miR390-dependent TAS sequences was identified in dicots.
These TAS loci are characterized by a smaller distance between miR390 sites compared to TAS3, a single copy of ta-siARF, and a sequence conservation pattern pointing to the possibility that processing of novel TAS-like locus is initiated by cleavage of the 5′-terminal miR390 target site.
American Psychological Association (APA)
Krasnikova, M. S.& Milyutina, I. A.& Bobrova, V. K.& Ozerova, L. V.& Troitsky, A. V.& Solovyev, A. G.…[et al.]. 2009. Novel miR390-Dependent Transacting siRNA Precursors in Plants Revealed by a PCR-Based Experimental Approach and Database Analysis. Journal of Biomedicine and Biotechnology،Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-510937
Modern Language Association (MLA)
Krasnikova, M. S.…[et al.]. Novel miR390-Dependent Transacting siRNA Precursors in Plants Revealed by a PCR-Based Experimental Approach and Database Analysis. Journal of Biomedicine and Biotechnology No. 2009 (2009), pp.1-9.
https://search.emarefa.net/detail/BIM-510937
American Medical Association (AMA)
Krasnikova, M. S.& Milyutina, I. A.& Bobrova, V. K.& Ozerova, L. V.& Troitsky, A. V.& Solovyev, A. G.…[et al.]. Novel miR390-Dependent Transacting siRNA Precursors in Plants Revealed by a PCR-Based Experimental Approach and Database Analysis. Journal of Biomedicine and Biotechnology. 2009. Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-510937
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-510937