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Chemical and Physical Methods to Analyze a Multicomponent Traditional Chinese Herbal Prescription Using LC-MSMS, Electron Microscope, and Congo Red Staining
Joint Authors
Lin, Lie-Chwen
Lu, Chia-Ming
Hou, Mei-Ling
Tsai, Tung-Hu
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-12
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
This study develops several chemical and physical methods to evaluate the quality of a traditional Chinese formulation, Jia-Wei-Xiao-Yao-San.
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) coupled with electrospray ionization was used to measure the herbal biomarkers of saikosaponin A, saikosaponin D, ferulic acid, and paeoniflorin from this herbal formula.
A scanning electron microscope (SEM) and light microscopy photographs with Congo red staining were used to identify the cellulose fibers if raw herbal powder had been added to the herbal pharmaceutical product.
Moreover, water solubility and crude fiber content examination were used to inspect for potential herbal additives to the herbal pharmaceutical products.
The results demonstrate that the contents of the herbal ingredients of saikosaponin A, saikosaponin D, ferulic acid, and paeoniflorin were around 0.351 ± 0.017, 0.136 ± 0.010, 0.140 ± 0.005, and 2.281 ± 0.406 mg/g, respectively, for this herbal pharmaceutical product.
The physical examination data demonstrate that the raw herbal powder had rough, irregular, lumpy, filamentous, and elongated shapes, as well as strong Congo red staining.
In addition, water solubility and crude fiber content were not consistent in the herbal pharmaceutical products.
American Psychological Association (APA)
Lu, Chia-Ming& Hou, Mei-Ling& Lin, Lie-Chwen& Tsai, Tung-Hu. 2013. Chemical and Physical Methods to Analyze a Multicomponent Traditional Chinese Herbal Prescription Using LC-MSMS, Electron Microscope, and Congo Red Staining. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-510988
Modern Language Association (MLA)
Lu, Chia-Ming…[et al.]. Chemical and Physical Methods to Analyze a Multicomponent Traditional Chinese Herbal Prescription Using LC-MSMS, Electron Microscope, and Congo Red Staining. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-510988
American Medical Association (AMA)
Lu, Chia-Ming& Hou, Mei-Ling& Lin, Lie-Chwen& Tsai, Tung-Hu. Chemical and Physical Methods to Analyze a Multicomponent Traditional Chinese Herbal Prescription Using LC-MSMS, Electron Microscope, and Congo Red Staining. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-510988
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-510988