Specific Intracellular Uptake of Herceptin-Conjugated CdSeZnS Quantum Dots into Breast Cancer Cells
Joint Authors
Moon, Jong-Sik
Lee, Joon-Hyung
Rathinaraj, Pierson
Winiarz, Jeffrey G.
Han, Seung-Jin
Park, Soo-Young
Kang, Inn-Kyu
Kim, Young Kyoo
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-09
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Herceptin, a typical monoclonal antibody, was immobilized on the surface of CdSe/ZnS core-shell quantum dots (QDs) to enhance their specific interactions with breast cancer cells (SK-BR3).
The mean size of the core-shell quantum dots (28 nm), as determined by dynamic light scattering, increased to 86 nm after herceptin immobilization.
The in vitro cell culture experiment showed that the keratin forming cancer cells (KB) proliferated well in the presence of herceptin-conjugated QDs (QD-Her, 5 nmol/mL), whereas most of the breast cancer cells (SK-BR3) had died.
To clarify the mechanism of cell death, the interaction of SK-BR3 cells with QD-Her was examined by confocal laser scanning microscopy.
As a result, the QD-Her bound specifically to the membrane of SK-BR3, which became almost saturated after 6 hours incubation.
This suggests that the growth signal of breast cancer cells is inhibited completely by the specific binding of herceptin to the Her-2 receptor of SK-BR3 membrane, resulting in cell death.
American Psychological Association (APA)
Han, Seung-Jin& Rathinaraj, Pierson& Park, Soo-Young& Kim, Young Kyoo& Lee, Joon-Hyung& Kang, Inn-Kyu…[et al.]. 2014. Specific Intracellular Uptake of Herceptin-Conjugated CdSeZnS Quantum Dots into Breast Cancer Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-511151
Modern Language Association (MLA)
Han, Seung-Jin…[et al.]. Specific Intracellular Uptake of Herceptin-Conjugated CdSeZnS Quantum Dots into Breast Cancer Cells. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-511151
American Medical Association (AMA)
Han, Seung-Jin& Rathinaraj, Pierson& Park, Soo-Young& Kim, Young Kyoo& Lee, Joon-Hyung& Kang, Inn-Kyu…[et al.]. Specific Intracellular Uptake of Herceptin-Conjugated CdSeZnS Quantum Dots into Breast Cancer Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-511151
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-511151