Extraction, identification and antibacterial activity of cyperus oil from iraqi c. rotundus

Abstract EN

Cyprus rotundas has many different uses and these were based on the different parts of plant.

The medical uses of cyperus have been used in medicine for thousands of years.

The parts of the cyperus used are its leaves, seeds and oil.

The Extraction process was carried out by steam distillation.

Optimum organic extractant determined.

The collected oil was identified via Thin Layer Chromatography (TLC) using a mixture of Ethyl acetate : toluene (1:9) as chromatographic eluent.

This study was designed to extract and identify of essential cyperus oil from C.

rotundus .The Antibacterial activity of Cyperus oil was studied for various microorganisms (Staphylococcus aureus , Klebsiella pneumoniae, Proteus vulgaris, Streptococcus pyogenes , Escherichia coli and Pseudomonas aeruginosa) using inhibition zone method (Aromatogram).

The MIC and MBC for each microbe were estimated.

The oil of C.

rotundus was shown a remarkable activity against gram-positive bacteria, less antibacterial activity was found against gram–negative bacteria and no activity was observed with the oil against Pseudomonas aeruginosa and Proteus vulgaris.

Novel method for extraction and identification of chemical composition for Iraqi cyperus oil was conducted.

The study of the biological activities of this oil is very important because of needing to be determined whether there is any correlation between the biological activities and one or more of the chemical compounds purified from C.

rotundus oil.