Effects of Dental Methacrylates on Oxygen Consumption and Redox Status of Human Pulp Cells
Joint Authors
Cordaro, Massimo
Cicillini, Loredana
Martorana, Giuseppe Ettore
Lupi, Alessandro
Luisa Gozzo, Maria
Callà, Cinzia Anna Maria
Rengo, Sandro
Nocca, Giuseppina
Spagnuolo, Gianrico
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-12
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Several studies have already demonstrated that the incomplete polymerization of resin-based dental materials causes the release of monomers which might affect cell metabolism.
The aim of this study was to investigate the effects of triethylene glycol dimethacrylate, 1,4-butanediol dimethacrylate, urethane dimethacrylate, and 2-hydroxyethyl methacrylate on (1) cellular energy metabolism, evaluating oxygen consumption rate, glucose consumption, glucose 6-phosphate dehydrogenase activity, and lactate production, and (2) cellular redox status, through the evaluation of glutathione concentration and of the activities of enzymes regulating glutathione metabolism.
Methods.
Human pulp cells were used and oxygen consumption was measured by means of a Clark electrode.
Moreover, reactive oxygen species production was quantified.
Enzymatic activity and glucose and lactate concentrations were determined through a specific kit.
Results.
Triethylene glycol dimethacrylate, 1,4-butanediol dimethacrylate, and 2-hydroxyethyl methacrylate induced a decrease in oxygen consumption rate, an enhancement of glucose consumption, and lactate production, whilst glucose 6-phosphate dehydrogenase and glutathione reductase activity were not significantly modified.
Moreover, the monomers induced an increase of reactive oxygen species production with a consequent increase of superoxide dismutase and catalase enzymatic activities.
A depletion of both reduced and total glutathione was also observed.
Conclusion.
The obtained results indicate that dental monomers might alter energy metabolism and glutathione redox balance in human pulp cells.
American Psychological Association (APA)
Nocca, Giuseppina& Callà, Cinzia Anna Maria& Martorana, Giuseppe Ettore& Cicillini, Loredana& Rengo, Sandro& Lupi, Alessandro…[et al.]. 2014. Effects of Dental Methacrylates on Oxygen Consumption and Redox Status of Human Pulp Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-511260
Modern Language Association (MLA)
Nocca, Giuseppina…[et al.]. Effects of Dental Methacrylates on Oxygen Consumption and Redox Status of Human Pulp Cells. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-511260
American Medical Association (AMA)
Nocca, Giuseppina& Callà, Cinzia Anna Maria& Martorana, Giuseppe Ettore& Cicillini, Loredana& Rengo, Sandro& Lupi, Alessandro…[et al.]. Effects of Dental Methacrylates on Oxygen Consumption and Redox Status of Human Pulp Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-511260
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-511260