Proteomic Analysis of Pichindé virus Infection Identifies Differential Expression of Prothymosin-α
Joint Authors
Soman, Kizhake V.
Gorenstein, David G.
Bowick, Gavin C.
Lomas, Lee O.
Herzog, Norbert K.
Wang, He
Luxon, Bruce A.
Aronson, Judith F.
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-08
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The arenaviruses include a number of important pathogens including Lassa virus and Junin virus.
Presently, the only treatment is supportive care and the antiviral Ribavirin.
In the event of an epidemic, patient triage may be required to more effectively manage resources; the development of prognostic biomarker signatures, correlating with disease severity, would allow rational triage.
Using a pair of arenaviruses, which cause mild or severe disease, we analyzed extracts from infected cells using SELDI mass spectrometry to characterize potential biomarker profiles.
EDGE analysis was used to analyze longitudinal expression differences.
Extracts from infected guinea pigs revealed protein peaks which could discriminate between mild or severe infection and between times post-infection.
Tandem mass-spectrometry identified several peaks, including the transcriptional regulator prothymosin-α.
Further investigation revealed differences in secretion of this peptide.
These data show proof of concept that proteomic profiling of host markers could be used as prognostic markers of infectious disease.
American Psychological Association (APA)
Bowick, Gavin C.& Soman, Kizhake V.& Wang, He& Aronson, Judith F.& Luxon, Bruce A.& Lomas, Lee O.…[et al.]. 2010. Proteomic Analysis of Pichindé virus Infection Identifies Differential Expression of Prothymosin-α. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-511274
Modern Language Association (MLA)
Bowick, Gavin C.…[et al.]. Proteomic Analysis of Pichindé virus Infection Identifies Differential Expression of Prothymosin-α. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-9.
https://search.emarefa.net/detail/BIM-511274
American Medical Association (AMA)
Bowick, Gavin C.& Soman, Kizhake V.& Wang, He& Aronson, Judith F.& Luxon, Bruce A.& Lomas, Lee O.…[et al.]. Proteomic Analysis of Pichindé virus Infection Identifies Differential Expression of Prothymosin-α. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-511274
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-511274