A Novel Sandwich Electrochemical Immunosensor Based on the DNA-Derived Magnetic Nanochain Probes for Alpha-Fetoprotein
Joint Authors
Jia, Liyong
Gan, Ning
Zheng, Lei
Source
Journal of Automated Methods and Management in Chemistry
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-13
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
One novel electrochemical immunosensor was constructed by immobilizing capture antibody of alpha-fetoprotein (AFP Ab1) on a nafion/nanogold-particle modified glassy carbon electrode.
With a sandwich immunoassay, one DNA-derived magnetic nanoprobe, simplified as DNA/(ZMPs—HRP-AFP Ab2)n, was employed for the detection of AFP.
The fabricated procedure of the proposed biosensor was characterized by cyclic voltammetry and electrochemical impedance spectroscopy.
The performance and factors influencing the performance of the biosensor were also evaluated.
Under optimal conditions, the developed biosensor exhibited a well-defined electrochemical behavior toward the reduction of AFP ranging from 0.01 to 200 ng/mL with a detection limit of 4 pg/mL (S/N=3).
The biosensor was applied to the determination of AFP in serum with satisfactory results.
It is important to note that the sandwich nanochainmodified electro-immunosensor provided an alternative substrate for the immobilization of other tumor markers.
American Psychological Association (APA)
Gan, Ning& Jia, Liyong& Zheng, Lei. 2011. A Novel Sandwich Electrochemical Immunosensor Based on the DNA-Derived Magnetic Nanochain Probes for Alpha-Fetoprotein. Journal of Automated Methods and Management in Chemistry،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-511381
Modern Language Association (MLA)
Gan, Ning…[et al.]. A Novel Sandwich Electrochemical Immunosensor Based on the DNA-Derived Magnetic Nanochain Probes for Alpha-Fetoprotein. Journal of Automated Methods and Management in Chemistry No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-511381
American Medical Association (AMA)
Gan, Ning& Jia, Liyong& Zheng, Lei. A Novel Sandwich Electrochemical Immunosensor Based on the DNA-Derived Magnetic Nanochain Probes for Alpha-Fetoprotein. Journal of Automated Methods and Management in Chemistry. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-511381
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-511381