Growth Inhibition and Induction of Apoptosis in SHG-44 Glioma Cells by Chinese Medicine Formula “Pingliu Keli”
Joint Authors
Lu, Wuguang
Zhou, Fei
Huo, Jiege
Cai, Xueting
Cao, Peng
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-09-08
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The present study was carried out to evaluate the effects of the water extract of Chinese medicine “Pingliu Keli” (PK) on human glioma cell viability and apoptosis and to investigate its mechanisms of action in SHG-44 cells.
MTT assay showed that PK had a strong cytotoxic effect on SHG-44 cells.
The number of live cells was less than 20% after exposure to 90 μg/mL PK for 24 h.
PK increased cytotoxicity of SHG-44 cells in a dose-dependent manner.
PK caused arrest of SHG-44 cells in G1 phase at low concentration and in G2 phase at high concentration.
The percentage of apoptotic cells by flow cytometric analysis of the DNA-stained cells increased to 38% and 52% after treatment with 72 and 108 μg/mL PK, respectively.
In addition, PK increased the expression of proapoptotic protein (Bax) and decreased antiapoptotic protein (Bcl-2), with a concomitant increase in the levels of cleaved caspase-3, cleaved caspase-9 and cleaved poly-ADP-ribose polymerase (PARP).
These results suggest that PK has a significant apoptosis inducing effect on SHG-44 glioma cells in vitro and caspase-3 may act as a potential mediator in the process.
American Psychological Association (APA)
Cao, Peng& Cai, Xueting& Lu, Wuguang& Zhou, Fei& Huo, Jiege. 2010. Growth Inhibition and Induction of Apoptosis in SHG-44 Glioma Cells by Chinese Medicine Formula “Pingliu Keli”. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-511421
Modern Language Association (MLA)
Cao, Peng…[et al.]. Growth Inhibition and Induction of Apoptosis in SHG-44 Glioma Cells by Chinese Medicine Formula “Pingliu Keli”. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-511421
American Medical Association (AMA)
Cao, Peng& Cai, Xueting& Lu, Wuguang& Zhou, Fei& Huo, Jiege. Growth Inhibition and Induction of Apoptosis in SHG-44 Glioma Cells by Chinese Medicine Formula “Pingliu Keli”. Evidence-Based Complementary and Alternative Medicine. 2010. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-511421
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-511421