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Identification of Virulence Factors Genes in Escherichia coli Isolates from Women with Urinary Tract Infection in Mexico
Joint Authors
Xicohtencatl-Cortes, Juan
Villanueva-Recillas, Silvia
Manjarrez-Hernández, Ángel H.
Arroyo-Escalante, Sara
López-Banda, Daniela A.
Hernández-Castro, Rigoberto
Leyva-Leyva, Margarita
Carrillo-Casas, Erika M.
Moncada-Barrón, David
Orozco-Hoyuela, Gabriel
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-05
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
E coli isolates (108) from Mexican women, clinically diagnosed with urinary tract infection, were screened to identify virulence genes, phylogenetic groups, and antibiotic resistance.
Isolates were identified by MicroScan4 system; additionally, the minimum inhibitory concentration (MIC) was assessed.
The phylogenetic groups and 16 virulence genes encoding adhesins, toxins, siderophores, lipopolysaccharide (LPS), and invasins were identified by PCR.
Phylogenetic groups distribution was as follows: B1 9.3%, A 30.6%, B2 55.6%, and D 4.6%.
Virulence genes prevalence was ecp 98.1%, fimH 86.1%, traT 77.8%, sfa/focDE 74.1%, papC 62%, iutA 48.1%, fyuA 44.4%, focG 2.8%, sfaS 1.9%, hlyA 7.4%, cnf-1 6.5%, cdt-B 0.9%, cvaC 2.8%, ibeA 2.8%, and rfc 0.9%.
Regarding antimicrobial resistance it was above 50% to ampicillin/sulbactam, ampicillin, piperacillin, trimethoprim/sulfamethoxazole, ciprofloxacin, and levofloxacin.
Uropathogenic E.
coli clustered mainly in the pathogenic phylogenetic group B2.
The isolates showed a high presence of siderophores and adhesion genes and a low presence of genes encoding toxins.
The high frequency of papC gene suggests that these isolates have the ability to colonize the kidneys.
High resistance to drugs considered as first choice treatment such as trimethoprim/sulfamethoxazole and fluoroquinolones was consistently observed.
American Psychological Association (APA)
López-Banda, Daniela A.& Carrillo-Casas, Erika M.& Leyva-Leyva, Margarita& Orozco-Hoyuela, Gabriel& Manjarrez-Hernández, Ángel H.& Arroyo-Escalante, Sara…[et al.]. 2014. Identification of Virulence Factors Genes in Escherichia coli Isolates from Women with Urinary Tract Infection in Mexico. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-511500
Modern Language Association (MLA)
López-Banda, Daniela A.…[et al.]. Identification of Virulence Factors Genes in Escherichia coli Isolates from Women with Urinary Tract Infection in Mexico. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-511500
American Medical Association (AMA)
López-Banda, Daniela A.& Carrillo-Casas, Erika M.& Leyva-Leyva, Margarita& Orozco-Hoyuela, Gabriel& Manjarrez-Hernández, Ángel H.& Arroyo-Escalante, Sara…[et al.]. Identification of Virulence Factors Genes in Escherichia coli Isolates from Women with Urinary Tract Infection in Mexico. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-511500
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-511500