Cell Surface Binding and Internalization of Aβ Modulated by Degree of Aggregation
Joint Authors
Bateman, David A.
Chakrabartty, Avijit
Source
International Journal of Alzheimer's Disease
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-01-24
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The amyloid peptides, Aβ40 and Aβ42, are generated through endoproteolytic cleavage of the amyloid precursor protein.
Here we have developed a model to investigate the interaction of living cells with various forms of aggregated Aβ40/42.
After incubation at endosomal pH 6, we observed a variety of Aβ conformations after 3 (Aβ3), 24 (Aβ24), and 90 hours (Aβ90).
Both Aβ4224 and Aβ4024 were observed to rapidly bind and internalize into differentiated PC12 cells, leading to accumulation in the lysosome.
In contrast, Aβ40/4290 were both found to only weakly associate with cells, but were observed as the most aggregated using dynamic light scattering and thioflavin-T.
Internalization of Aβ40/4224 was inhibited with treatment of monodansylcadaverine, an endocytosis inhibitor.
These studies indicate that the ability of Aβ40/42 to bind and internalize into living cells increases with degree of aggregation until it reaches a maximum beyond which its ability to interact with cells diminishes drastically.
American Psychological Association (APA)
Bateman, David A.& Chakrabartty, Avijit. 2011. Cell Surface Binding and Internalization of Aβ Modulated by Degree of Aggregation. International Journal of Alzheimer's Disease،Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-511767
Modern Language Association (MLA)
Bateman, David A.& Chakrabartty, Avijit. Cell Surface Binding and Internalization of Aβ Modulated by Degree of Aggregation. International Journal of Alzheimer's Disease No. 2011 (2011), pp.1-13.
https://search.emarefa.net/detail/BIM-511767
American Medical Association (AMA)
Bateman, David A.& Chakrabartty, Avijit. Cell Surface Binding and Internalization of Aβ Modulated by Degree of Aggregation. International Journal of Alzheimer's Disease. 2011. Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-511767
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-511767