Effects of Endurance and Resistance Training on Calcitonin Gene-Related Peptide and Acetylcholine Receptor at Slow and Fast Twitch Skeletal Muscles and Sciatic Nerve in Male Wistar Rats

Abstract EN

The aim of this study was to investigate effects of endurance and resistance training (ET and RT) on CGRP and AChRs at slow and fast twitch muscles and sciatic nerve in rats.

Twenty-five male rats were randomly assigned into three groups including sedentary (SED), endurance training (ET), and resistance training (RT).

Animals of ET exercised for 12 weeks, five times/week, and 60 min/day at 30 m/min.

Animals of RT were housed in metal cage with 2 m high wire-mesh tower, with water bottles set at the top.

48 h after the last session of training protocol, animals were anaesthetized.

The right sciatic nerves were removed; then, Soleus (SOL) and Tibialis anterior (TA) muscles were excised and immediately snap frozen in liquid nitrogen.

All frozen tissues were stored at −80°C.

Results showed that, after both ET and RT, CGRP content as well as AChR content of SOL and TA muscles significantly increased.

But there was no significant difference among groups at sciatic nerve’ CGRP content.

In conclusion, data demonstrate that ET and RT lead to changes of CGRP and AChR content of ST and FT muscles.

The changes indicate to the importance of neuromuscular activity.