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Preparation of a Nanoscaled Poly(vinyl alcohol)HydroxyapatiteDNA Complex Using High Hydrostatic Pressure Technology for In Vitro and In Vivo Gene Delivery
Joint Authors
Furuzono, Tsutomu
Ono, Tsutomu
Okada, Masahiro
Kimura, Tsuyoshi
Nam, Kwangwoo
Fujisato, Toshiya
Funamoto, Seiichi
Yoshizawa, Hidekazu
Nibe, Yoichi
Kishida, Akio
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-12
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Our previous research showed that poly(vinyl alcohol) (PVA) nanoparticles incorporating DNA with hydrogen bonds obtained by high hydrostatic pressurization are able to deliver DNA without any significant cytotoxicity.
To enhance transfection efficiency of PVA/DNA nanoparticles, we describe a novel method to prepare PVA/DNA nanoparticles encapsulating nanoscaled hydroxyapatites (HAps) prepared by high hydrostatic pressurization (980 MPa), which is designed to facilitate endosomal escape induced by dissolving HAps in an endosome.
Scanning electron microscopic observation and dynamic light scattering measurement revealed that HAps were significantly encapsulated in PVA/HAp/DNA nanoparticles.
The cytotoxicity, cellular uptake, and transgene expression of PVA/HAp/DNA nanoparticles were investigated using COS-7 cells.
It was found that, in contrast to PVA/DNA nanoparticles, their internalization and transgene expression increased without cytotoxicity occurring.
Furthermore, a similar level of transgene expression between plasmid DNA and PVA/HAp/DNA nanoparticles was achieved using in vivo hydrodynamic injection.
Our results show a novel method of preparing PVA/DNA nanoparticles encapsulating HAp nano-crystals by using high hydrostatic pressure technology and the potential use of HAps as an enhancer of the transfection efficiency of PVA/DNA nanoparticles without significant cytotoxicity.
American Psychological Association (APA)
Kimura, Tsuyoshi& Nibe, Yoichi& Funamoto, Seiichi& Okada, Masahiro& Furuzono, Tsutomu& Ono, Tsutomu…[et al.]. 2011. Preparation of a Nanoscaled Poly(vinyl alcohol)HydroxyapatiteDNA Complex Using High Hydrostatic Pressure Technology for In Vitro and In Vivo Gene Delivery. Journal of Drug Delivery،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-511805
Modern Language Association (MLA)
Kimura, Tsuyoshi…[et al.]. Preparation of a Nanoscaled Poly(vinyl alcohol)HydroxyapatiteDNA Complex Using High Hydrostatic Pressure Technology for In Vitro and In Vivo Gene Delivery. Journal of Drug Delivery No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-511805
American Medical Association (AMA)
Kimura, Tsuyoshi& Nibe, Yoichi& Funamoto, Seiichi& Okada, Masahiro& Furuzono, Tsutomu& Ono, Tsutomu…[et al.]. Preparation of a Nanoscaled Poly(vinyl alcohol)HydroxyapatiteDNA Complex Using High Hydrostatic Pressure Technology for In Vitro and In Vivo Gene Delivery. Journal of Drug Delivery. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-511805
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-511805