Human Serum Albumin Conjugates of 7-Ethyl-10-hydroxycamptothecin (SN38) for Cancer Treatment
Joint Authors
Ghahremani, Mohammad Hossein
Rouhani, Hasti
Ghanbarpour, Ahmad Reza
Dinarvand, Rassoul
Tavassolian, Faranak
Gharghabi, Mehdi
Ostad, Seyed Nasser
Sepehri, Nima
Amini, Mohsen
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-07
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
SN38 (7-ethyl-10-hydroxy-comptothecin) is a potent metabolite of irinotecan, which has been approved for treatment of metastatic colorectal cancer.
Considering the notable potency of SN38, it has been introduced as an anticancer candidate.
In this study, human serum albumin (HSA) conjugates of SN38 were formulated to overcome the solubility problem beside improving the active form stability and tumor tissue targeting.
In this target, two different molar ratios of conjugates (SN38 : HSA 15 : 1 and 60 : 1) were prepared by derivatization of 20-hydroxyl group of SN38 with glycine, followed by addition of succinyl group to glycine through which HSA was covalently attached.
The conjugates with particle size of about 100 nm revealed enhanced water solubility and were relatively stable in neutral and acidic solutions.
For SN38-HSA-15 and SN38-HSA-60 IC50 values were compared with irinotecan in HT-29 human colon cancer cells.
Furthermore, biodistribution studies of SN38-HSA conjugate resulted in proper blood concentration level within 4 h.
Moreover, blood cytotoxicity assay revealed no toxicity effect on liver and spleen.
Collectively, our present investigation offers a water-soluble form of SN38 attached to HSA and suggests using favorable properties as a promising anticancer agent for further preclinical and clinical investigations.
American Psychological Association (APA)
Sepehri, Nima& Rouhani, Hasti& Ghanbarpour, Ahmad Reza& Gharghabi, Mehdi& Tavassolian, Faranak& Amini, Mohsen…[et al.]. 2014. Human Serum Albumin Conjugates of 7-Ethyl-10-hydroxycamptothecin (SN38) for Cancer Treatment. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-511866
Modern Language Association (MLA)
Sepehri, Nima…[et al.]. Human Serum Albumin Conjugates of 7-Ethyl-10-hydroxycamptothecin (SN38) for Cancer Treatment. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-511866
American Medical Association (AMA)
Sepehri, Nima& Rouhani, Hasti& Ghanbarpour, Ahmad Reza& Gharghabi, Mehdi& Tavassolian, Faranak& Amini, Mohsen…[et al.]. Human Serum Albumin Conjugates of 7-Ethyl-10-hydroxycamptothecin (SN38) for Cancer Treatment. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-511866
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-511866