Innate Immunity in Lobsters : Partial Purification and Characterization of a Panulirus cygnus Anti-A Lectin
Author
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-05
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
A lectin detected in haemolymph from the Australian spiny lobster Panulirus cygnus agglutinated human ABO Group A cells to a higher titre than Group O or B.
The lectin also agglutinated rat and sheep erythrocytes, with reactivity with rat erythrocytes strongly enhanced by treatment with the proteolytic enzyme papain, an observation consistent with reactivity via a glycolipid.
The lectin, purified by affinity chromatography on fixed rat-erythrocyte stroma, was inhibited equally by N-acetylglucosamine and N-acetylgalactosamine.
Comparison of data from gel filtration of haemolymph (behaving as a 1,800,000 Da macromolecule), and polyacrylamide gel electrophoresis of purified lectin (a single 67,000 Da band), suggested that in haemolymph the lecin was a multimer.
The purified anti-A lectin autoprecipitated unless the storage solution contained chaotropic inhibitors (125 mmol/L sucrose: 500 mmol/L urea).
The properties of this anti-A lectin and other similar lectins are consistent with a role in innate immunity in these invertebrates.
American Psychological Association (APA)
Flower, Robert L. P.. 2012. Innate Immunity in Lobsters : Partial Purification and Characterization of a Panulirus cygnus Anti-A Lectin. ISRN Hematology،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-511998
Modern Language Association (MLA)
Flower, Robert L. P.. Innate Immunity in Lobsters : Partial Purification and Characterization of a Panulirus cygnus Anti-A Lectin. ISRN Hematology No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-511998
American Medical Association (AMA)
Flower, Robert L. P.. Innate Immunity in Lobsters : Partial Purification and Characterization of a Panulirus cygnus Anti-A Lectin. ISRN Hematology. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-511998
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-511998