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Escherichia coli ATCC 8739 Adapts to the Presence of Sodium Chloride, Monosodium Glutamate, and Benzoic Acid after Extended Culture
Joint Authors
Lee, Chin How
Ling, Maurice H. T.
Oon, Jack S. H.
Lee, Kun Cheng
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-05
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Escherichia coli is commonly found in intestine of human, and any changes in their adaptation or evolution may affect the human body.
The relationship between E.
coli and food additives is less studied as compared to antibiotics.
E.
coli within our human gut are consistently interacting with the food additives; thus, it is important to investigate this relationship.
In this paper, we observed the evolution of E.
coli cultured in different concentration of food additives (sodium chloride, benzoic acid, and monosodium glutamate), singly or in combination, over 70 passages.
Adaptability over time was estimated by generation time and cell density at stationary phase.
Polymerase chain reaction (PCR)/restriction fragments length polymorphism (RFLP) using 3 primers and restriction endonucleases, each was used to characterize adaptation/evolution at genomic level.
The amplification and digestion profiles were tabulated and analyzed by Nei-Li dissimilarity index.
Our results demonstrate that E.
coli in every treatment had adapted over 465 generations.
The types of stress were discovered to be different even though different concentrations of same additives were used.
However, RFLP shows a convergence of genetic distances, suggesting the presence of global stress response.
In addition, monosodium glutamate may be a nutrient source and support acid resistance in E.
coli.
American Psychological Association (APA)
Lee, Chin How& Oon, Jack S. H.& Lee, Kun Cheng& Ling, Maurice H. T.. 2012. Escherichia coli ATCC 8739 Adapts to the Presence of Sodium Chloride, Monosodium Glutamate, and Benzoic Acid after Extended Culture. ISRN Microbiology،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-512039
Modern Language Association (MLA)
Lee, Chin How…[et al.]. Escherichia coli ATCC 8739 Adapts to the Presence of Sodium Chloride, Monosodium Glutamate, and Benzoic Acid after Extended Culture. ISRN Microbiology No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-512039
American Medical Association (AMA)
Lee, Chin How& Oon, Jack S. H.& Lee, Kun Cheng& Ling, Maurice H. T.. Escherichia coli ATCC 8739 Adapts to the Presence of Sodium Chloride, Monosodium Glutamate, and Benzoic Acid after Extended Culture. ISRN Microbiology. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-512039
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-512039