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Breast Carcinoma Cells in Primary Tumors and Effusions Have Different Gene Array Profiles
Joint Authors
Reich, Reuven
Davidson, Ben
Smith, Yoav
Tuft Stavnes, Helene
Zilber, Sofia
Nesland, Jahn M.
Konstantinovsky, Sophya
Becker, Anne-Marie
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-08-11
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
The detection of breast carcinoma cells in effusions is associated with rapidly fatal outcome, but these cells are poorly characterized at the molecular level.
This study compared the gene array signatures of breast carcinoma cells in primary carcinomas and effusions.
The genetic signature of 10 primary tumors and 10 effusions was analyzed using the Array-Ready Oligo set for the Human Genome platform.
Results for selected genes were validated using PCR, Western blotting, and immunohistochemistry.
Array analysis identified 255 significantly downregulated and 96 upregulated genes in the effusion samples.
The majority of differentially expressed genes were part of pathways involved in focal adhesion, extracellular matrix-cell interaction, and the regulation of the actin cytoskeleton.
Genes that were upregulated in effusions included KRT8, BCAR1, CLDN4, VIL2, while DCN, CLDN19, ITGA7, and ITGA5 were downregulated at this anatomic site.
PCR, Western blotting, and immunohistochemistry confirmed the array findings for BCAR1, CLDN4, VIL2, and DCN.
Our data show that breast carcinoma cells in primary carcinomas and effusions have different gene expression signatures, and differentially express a large number of molecules related to adhesion, motility, and metastasis.
These differences may have a critical role in designing therapy and in prognostication for patients with metastatic disease localized to the serosal cavities.
American Psychological Association (APA)
Konstantinovsky, Sophya& Smith, Yoav& Zilber, Sofia& Tuft Stavnes, Helene& Becker, Anne-Marie& Nesland, Jahn M.…[et al.]. 2009. Breast Carcinoma Cells in Primary Tumors and Effusions Have Different Gene Array Profiles. Journal of Oncology،Vol. 2010, no. 2010, pp.1-14.
https://search.emarefa.net/detail/BIM-512251
Modern Language Association (MLA)
Konstantinovsky, Sophya…[et al.]. Breast Carcinoma Cells in Primary Tumors and Effusions Have Different Gene Array Profiles. Journal of Oncology No. 2010 (2010), pp.1-14.
https://search.emarefa.net/detail/BIM-512251
American Medical Association (AMA)
Konstantinovsky, Sophya& Smith, Yoav& Zilber, Sofia& Tuft Stavnes, Helene& Becker, Anne-Marie& Nesland, Jahn M.…[et al.]. Breast Carcinoma Cells in Primary Tumors and Effusions Have Different Gene Array Profiles. Journal of Oncology. 2009. Vol. 2010, no. 2010, pp.1-14.
https://search.emarefa.net/detail/BIM-512251
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-512251