Electromagnetic Fields Effects on the Secondary Structure of Lysozyme and Bioprotective Effectiveness of Trehalose
Joint Authors
Magazù, Salvatore
Calabrò, Emanuele
Source
Advances in Physical Chemistry
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-08-09
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
FTIR spectroscopy was used to investigate the effects of extremely low frequency (50 Hz) electromagnetic field and of microwaves at 900 MHz on the secondary structure of a typical protein, the lysozyme, evaluating the bioprotective effectiveness of trehalose.
Lysozyme in D2O solution (60 mg/ml) was exposed to 50 Hz frequency electromagnetic field at 180 μT.
The FTIR spectra indicated an increase of CH2 group at 1921 and 1853 cm−1 after 3 h of exposure.
Such effect was not observed after the addition of trehalose (150 mg/mL) at the same exposure conditions.
Lysozyme dissolved in D2O at the concentration of 100 mg/mL was exposed up to 4 h to 900 MHz mobile phone microwaves at 25 mA/m.
A significant increase in intensity of the amide I vibration band in the secondary structure of the protein was observed after 4 h exposure to microwaves.
This effect was inhibited by the presence of trehalose at the concentration of 150 mg/mL.
Fourier self-deconvolution spectral analysis of lysozyme in D2O solution after exposure to microwaves revealed an increase in intensity of the conformational components of amide I mode, particularly of β-sheet and turn that can be attributed to disorder and unfolding processes of the protein.
American Psychological Association (APA)
Calabrò, Emanuele& Magazù, Salvatore. 2012. Electromagnetic Fields Effects on the Secondary Structure of Lysozyme and Bioprotective Effectiveness of Trehalose. Advances in Physical Chemistry،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-512340
Modern Language Association (MLA)
Calabrò, Emanuele& Magazù, Salvatore. Electromagnetic Fields Effects on the Secondary Structure of Lysozyme and Bioprotective Effectiveness of Trehalose. Advances in Physical Chemistry No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-512340
American Medical Association (AMA)
Calabrò, Emanuele& Magazù, Salvatore. Electromagnetic Fields Effects on the Secondary Structure of Lysozyme and Bioprotective Effectiveness of Trehalose. Advances in Physical Chemistry. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-512340
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-512340