Isoflurane Enhances the Moonlighting Activity of GAPDH : Implications for GABAA Receptor Trafficking
Joint Authors
Fibuch, Eugene E.
Montalbano, Andrew J.
Seidler, Norbert W.
Theisen, Christopher S.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-17
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
GABAA receptor activity is directly modulated by glyceraldehyde 3-phosphate dehydrogenase (GAPDH), a protein with many nonglycolytic moonlighting functions.
In addition to playing a role in the phosphorylation of the receptor, GAPDH may also participate in proper receptor trafficking to the plasma membrane.
We previously showed that volatile anesthetics affect GAPDH structure and function that may contribute to the manner by which GAPDH modulates the GABAA receptor.
In the current study, GAPDH interacted with engineered phospholipid-containing vesicles, preferring association with phosphatidylserine over phosphatidylcholine.
Phosphatidyl-serine is known to participate in membrane trafficking of transport proteins and to play a role in GABAA receptor stability and function.
We observed that GAPDH promoted the self-association and fusion of phosphatidyl-serine-rich vesicles as well as decreased membrane fluidity.
Isoflurane enhanced each of these GAPDH-mediated events.
Isoflurane also increased the binding of GAPDH to the cytoplasmic loop of the GABAA receptor.
These observations are consistent with the working model of isoflurane playing a role in the trafficking of membrane proteins.
This study is the first to implicate GAPDH and isoflurane in the regulation of GABAA receptor localization, providing insight into the mechanism of action of anesthesia.
American Psychological Association (APA)
Montalbano, Andrew J.& Theisen, Christopher S.& Fibuch, Eugene E.& Seidler, Norbert W.. 2012. Isoflurane Enhances the Moonlighting Activity of GAPDH : Implications for GABAA Receptor Trafficking. ISRN Anesthesiology،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-512392
Modern Language Association (MLA)
Montalbano, Andrew J.…[et al.]. Isoflurane Enhances the Moonlighting Activity of GAPDH : Implications for GABAA Receptor Trafficking. ISRN Anesthesiology No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-512392
American Medical Association (AMA)
Montalbano, Andrew J.& Theisen, Christopher S.& Fibuch, Eugene E.& Seidler, Norbert W.. Isoflurane Enhances the Moonlighting Activity of GAPDH : Implications for GABAA Receptor Trafficking. ISRN Anesthesiology. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-512392
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-512392