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Bacterial Artificial Chromosome Mutagenesis Using Recombineering
Joint Authors
Chen, Qingwen
Narayanan, Kumaran
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-12-09
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Gene expression from bacterial artificial chromosome (BAC) clones has been demonstrated to facilitate physiologically relevant levels compared to viral and nonviral cDNA vectors.
BACs are large enough to transfer intact genes in their native chromosomal setting together with flanking regulatory elements to provide all the signals for correct spatiotemporal gene expression.
Until recently, the use of BACs for functional studies has been limited because their large size has inherently presented a major obstacle for introducing modifications using conventional genetic engineering strategies.
The development of in vivo homologous recombination strategies based on recombineering in E.
coli has helped resolve this problem by enabling facile engineering of high molecular weight BAC DNA without dependence on suitably placed restriction enzymes or cloning steps.
These techniques have considerably expanded the possibilities for studying functional genetics using BACs in vitro and in vivo.
American Psychological Association (APA)
Narayanan, Kumaran& Chen, Qingwen. 2010. Bacterial Artificial Chromosome Mutagenesis Using Recombineering. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-512439
Modern Language Association (MLA)
Narayanan, Kumaran& Chen, Qingwen. Bacterial Artificial Chromosome Mutagenesis Using Recombineering. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-512439
American Medical Association (AMA)
Narayanan, Kumaran& Chen, Qingwen. Bacterial Artificial Chromosome Mutagenesis Using Recombineering. Journal of Biomedicine and Biotechnology. 2010. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-512439
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-512439