Novel Bifunctional Single-Chain Variable Antibody Fragments to Enhance Virolysis by Complement : Generation and Proof-of-Concept
Joint Authors
Huber, Georg
Bánki, Zoltán
Kunert, Renate
Stoiber, Heribert
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-12
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
When bound to the envelope of viruses, factor H (FH), a soluble regulator of complement activation, contributes to the protection against a potent immune defense mechanism, the complement-mediated lysis (CML).
Thus, removing FH from the surface renders viruses, such as HIV, susceptible to CML.
For a proof of concept, we developed a construct consisting of recombinant bifunctional single-chain variable fragment (scFv) based on a monoclonal antibody against Friend murine leukemia virus (F-MuLV) envelope protein gp70, which was coupled to specific binding domains (short consensus repeats 19-20; SCR1920) of FH.
We used Pichia pastoris as expression system in common shake flasks and optimized expression in high density bench top fermentation.
Specific binding of recombinant scFv was proven by flow cytometry.
The recombinant scFv-SCR significantly enhanced CML of F-MuLV in vitro implying that FH binding to the viral surface was impaired by the scFv-SCR.
This novel concept to enhance virolysis may provide a new approach for antiviral treatment.
American Psychological Association (APA)
Huber, Georg& Bánki, Zoltán& Kunert, Renate& Stoiber, Heribert. 2014. Novel Bifunctional Single-Chain Variable Antibody Fragments to Enhance Virolysis by Complement : Generation and Proof-of-Concept. BioMed Research International،Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-512443
Modern Language Association (MLA)
Huber, Georg…[et al.]. Novel Bifunctional Single-Chain Variable Antibody Fragments to Enhance Virolysis by Complement : Generation and Proof-of-Concept. BioMed Research International No. 2014 (2014), pp.1-14.
https://search.emarefa.net/detail/BIM-512443
American Medical Association (AMA)
Huber, Georg& Bánki, Zoltán& Kunert, Renate& Stoiber, Heribert. Novel Bifunctional Single-Chain Variable Antibody Fragments to Enhance Virolysis by Complement : Generation and Proof-of-Concept. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-512443
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-512443