Proteolytic Potential of the MSC Exosome Proteome : Implications for an Exosome-Mediated Delivery of Therapeutic Proteasome
Joint Authors
Choo, Andre
Lim, Sai Kiang
Teh, Bao Ju
Arslan, Fatih
Lai, Ruenn Chai
de Kleijn, Dominique P.
Sze, Siu Kwan
Tan, Soon Sim
Source
International Journal of Proteomics
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-18
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Mesenchymal stem cells (MSCs) are used in many of the current stem cell-based clinical trials and their therapeutic efficacy has increasingly been attributed to secretion of paracrine factors.
We have previously demonstrated that a therapeutic constituent of this secretion is exosome, a secreted bilipid membrane vesicle of ~50–100 nm with a complex cargo that is readily internalized by H9C2 cardiomyocytes.
It reduces infarct size in a mouse model of myocardial ischemia/reperfusion (MI/R) injury.
We postulate that this therapeutic efficacy is derived from the synergy of a select permutation of individual exosome components.
To identify protein candidates in this permutation, the proteome was profiled and here we identified 20S proteasome as a protein candidate.
Mass spectrometry analysis detected all seven α and seven β chains of the 20S proteasome, and also the three beta subunits of “immunoproteasome” with a very high confidence level.
We demonstrated that a functional proteasome copurified with MSC exosomes with a density of 1.10–1.18 g/mL, and its presence correlated with a modest but significant reduction in oligomerized protein in a mouse model of myocardial infarction.
Circulating proteasomes in human blood also copurified with exosomes.
Therefore, 20S proteasome is a candidate exosome protein that could synergize with other constituents to ameliorate tissue damage.
American Psychological Association (APA)
Lai, Ruenn Chai& Tan, Soon Sim& Teh, Bao Ju& Sze, Siu Kwan& Arslan, Fatih& de Kleijn, Dominique P.…[et al.]. 2012. Proteolytic Potential of the MSC Exosome Proteome : Implications for an Exosome-Mediated Delivery of Therapeutic Proteasome. International Journal of Proteomics،Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-512499
Modern Language Association (MLA)
Lai, Ruenn Chai…[et al.]. Proteolytic Potential of the MSC Exosome Proteome : Implications for an Exosome-Mediated Delivery of Therapeutic Proteasome. International Journal of Proteomics No. 2012 (2012), pp.1-14.
https://search.emarefa.net/detail/BIM-512499
American Medical Association (AMA)
Lai, Ruenn Chai& Tan, Soon Sim& Teh, Bao Ju& Sze, Siu Kwan& Arslan, Fatih& de Kleijn, Dominique P.…[et al.]. Proteolytic Potential of the MSC Exosome Proteome : Implications for an Exosome-Mediated Delivery of Therapeutic Proteasome. International Journal of Proteomics. 2012. Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-512499
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-512499