Isolation and Expression Analysis of Novel Silicon Absorption Gene from Roots of Mangrove (Rhizophora apiculata) via Suppression Subtractive Hybridization
Joint Authors
Hanafi, Mohamed M.
Sahebi, Mahbod
Nejat, Naghmeh
Azizi, Parisa
Rafii, Mohd Y.
Idris, Abu Seman
Abdullah, Siti Nor Akmar
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-01
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Silicon (Si) is the second most abundant element in soil after oxygen.
It is not an essential element for plant growth and formation but plays an important role in increasing plant tolerance towards different kinds of abiotic and biotic stresses.
The molecular mechanism of Si absorption and accumulation may differ between plants, such as monocotyledons and dicotyledons.
Silicon absorption and accumulation in mangrove plants are affected indirectly by some proteins rich in serine and proline amino acids.
The expression level of the genes responsible for Si absorption varies in different parts of plants.
In this study, Si is mainly observed in the epidermal roots’ cell walls of mangrove plants compared to other parts.
The present work was carried out to discover further information on Si stress responsive genes in Rhizophora apiculata, using the suppression subtractive hybridization technique.
To construct the cDNA library, two-month-old seedlings were exposed to 0.5, 1, and 1.5 mM SiO2 for 15 hrs and for 1 to 6 days resulting in a total of 360 high quality ESTs gained.
Further examination by RT-PCR and real-time qRT-PCR showed the expression of a candidate gene of serine-rich protein.
American Psychological Association (APA)
Sahebi, Mahbod& Hanafi, Mohamed M.& Abdullah, Siti Nor Akmar& Rafii, Mohd Y.& Azizi, Parisa& Nejat, Naghmeh…[et al.]. 2014. Isolation and Expression Analysis of Novel Silicon Absorption Gene from Roots of Mangrove (Rhizophora apiculata) via Suppression Subtractive Hybridization. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-512506
Modern Language Association (MLA)
Sahebi, Mahbod…[et al.]. Isolation and Expression Analysis of Novel Silicon Absorption Gene from Roots of Mangrove (Rhizophora apiculata) via Suppression Subtractive Hybridization. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-512506
American Medical Association (AMA)
Sahebi, Mahbod& Hanafi, Mohamed M.& Abdullah, Siti Nor Akmar& Rafii, Mohd Y.& Azizi, Parisa& Nejat, Naghmeh…[et al.]. Isolation and Expression Analysis of Novel Silicon Absorption Gene from Roots of Mangrove (Rhizophora apiculata) via Suppression Subtractive Hybridization. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-512506
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-512506