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Comparison of Two Inoculation Methods for Evaluating Maize for Resistance to Aspergillus flavus Infection and Aflatoxin Accumulation
Joint Authors
Alpe, Michael N.
Mylroie, J. Erik
Ozkan, Seval
Windham, Gary L.
Williams, W. Paul
Source
International Journal of Agronomy
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-28
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Aflatoxin, the most potent carcinogen found in nature, is produced by the fungus Aspergillus flavus and occurs naturally in maize, Zea mays L.
Growing maize hybrids with genetic resistance to aflatoxin contamination are generally considered a highly desirable way to reduce losses to aflatoxin.
Developing resistant hybrids requires reliable inoculation methods for screening maize germplasm for resistance to A.
flavus infection and aflatoxin accumulation.
The side-needle technique is a widely used inoculation technique: an A.
flavus conidial suspension is injected underneath the husks into the side of the ear.
This wounds the ear and limits expression of resistance associated with husk coverage, pericarp thickness, and seed coat integrity.
In this investigation, the side-needle technique was compared with a second inoculation method that involved dispensing wheat kernels infected with A.
flavus into plant whorls at 35 and 49 days after planting.
Results showed that although the side-needle technique produced higher levels of aflatoxin accumulation, differences in A.
flavus biomass produced by the two inoculation techniques were not significant.
Both inoculation techniques were effective in differentiating resistant and susceptible single cross hybrids irrespective of the use of A.
flavus infection or aflatoxin accumulation as a basis to define resistance.
American Psychological Association (APA)
Williams, W. Paul& Alpe, Michael N.& Windham, Gary L.& Ozkan, Seval& Mylroie, J. Erik. 2013. Comparison of Two Inoculation Methods for Evaluating Maize for Resistance to Aspergillus flavus Infection and Aflatoxin Accumulation. International Journal of Agronomy،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-512528
Modern Language Association (MLA)
Williams, W. Paul…[et al.]. Comparison of Two Inoculation Methods for Evaluating Maize for Resistance to Aspergillus flavus Infection and Aflatoxin Accumulation. International Journal of Agronomy No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-512528
American Medical Association (AMA)
Williams, W. Paul& Alpe, Michael N.& Windham, Gary L.& Ozkan, Seval& Mylroie, J. Erik. Comparison of Two Inoculation Methods for Evaluating Maize for Resistance to Aspergillus flavus Infection and Aflatoxin Accumulation. International Journal of Agronomy. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-512528
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-512528