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Identification of Immunomodulatory Signatures Induced by American Ginseng in Murine Immune Cells
Joint Authors
Ma, Yonghui
Zhao, Fusheng
Jiao, Yan
Gu, Weikuan
Yan, Jian
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-11
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Background.
American ginseng (Panax quinquefolius, AG) has been used for more than 300 years.
Some of its claimed benefits can be attributed to the immunomodulatory activities, whose molecular mechanisms are largely unknown.
Methods.
Murine splenic cells from adult male C57BL/6 (B6) mice were isolated and divided into 4 groups to mimic 4 basic pathophysiological states: (1) normal naïve; (2) normal activated; (3) deficient naïve; (4) deficient activated.
Then, different AG extracts were added to all groups for 24 h incubation.
MTT proliferation assays were performed to evaluate the phenotypic features of cells.
Finally, microarray assays were carried out to identify differentially expressed genes associated with AG exposure.
Real-time PCR was performed to validate the expression of selected genes.
Results.
Microarray data showed that most of gene expression changes were identified in the deficient naïve group, suggesting that the pathophysiological state has major impacts on transcriptomic changes associated with AG exposure.
Specifically, this study revealed downregulation of interferon-γ signaling pathway in the deficient group of cells.
Conclusion.
Our study demonstrated that only specific groups of immune cells responded to AG intervention and immunocompromised cells were more likely regulated by AG treatment.
American Psychological Association (APA)
Yan, Jian& Ma, Yonghui& Zhao, Fusheng& Gu, Weikuan& Jiao, Yan. 2013. Identification of Immunomodulatory Signatures Induced by American Ginseng in Murine Immune Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-512598
Modern Language Association (MLA)
Zhao, Fusheng…[et al.]. Identification of Immunomodulatory Signatures Induced by American Ginseng in Murine Immune Cells. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-512598
American Medical Association (AMA)
Yan, Jian& Ma, Yonghui& Zhao, Fusheng& Gu, Weikuan& Jiao, Yan. Identification of Immunomodulatory Signatures Induced by American Ginseng in Murine Immune Cells. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-512598
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-512598