BAC-Dkk3-EGFP Transgenic Mouse : An In Vivo Analytical Tool for Dkk3 Expression
Joint Authors
Furukawa, Takahisa
Muranishi, Yuki
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Dickkopf (DKK) family proteins are secreted modulators of the Wnt signaling pathway and are capable of regulating the development of many organs and tissues.
We previously identified Dkk3 to be a molecule predominantly expressed in the mouse embryonic retina.
However, which cell expresses Dkk3 in the developing and mature mouse retina remains to be elucidated.
To examine the precise expression of the Dkk3 protein, we generated BAC-Dkk3-EGFP transgenic mice that express EGFP integrated into the Dkk3 gene in a BAC plasmid.
Expression analysis using the BAC-Dkk3-EGFP transgenic mice revealed that Dkk3 is expressed in retinal progenitor cells (RPCs) at embryonic stages and in Müller glial cells in the adult retina.
Since Müller glial cells may play a potential role in retinal regeneration, BAC-Dkk3-EGFP mice could be useful for retinal regeneration studies.
American Psychological Association (APA)
Muranishi, Yuki& Furukawa, Takahisa. 2012. BAC-Dkk3-EGFP Transgenic Mouse : An In Vivo Analytical Tool for Dkk3 Expression. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-512630
Modern Language Association (MLA)
Muranishi, Yuki& Furukawa, Takahisa. BAC-Dkk3-EGFP Transgenic Mouse : An In Vivo Analytical Tool for Dkk3 Expression. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-512630
American Medical Association (AMA)
Muranishi, Yuki& Furukawa, Takahisa. BAC-Dkk3-EGFP Transgenic Mouse : An In Vivo Analytical Tool for Dkk3 Expression. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-512630
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-512630