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Estimation of the Postmortem Duration of Mouse Tissue by Electron Spin Resonance Spectroscopy
Joint Authors
Mori, Tomohisa
Ito, Shinobu
Kanazawa, Hideko
Sawaguchi, Toshiko
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-06-27
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Pharmacy, Health & Medical Sciences
Medicine
Abstract EN
Electron spin resonance (ESR) method is a simple method for detecting various free radicals simultaneously and directly.
However, ESR spin trap method is unsuited to analyze weak ESR signals in organs because of water-induced dielectric loss (WIDL).
To minimize WIDL occurring in biotissues and to improve detection sensitivity to free radicals in tissues, ESR cuvette was modified and used with 5,5-dimethtyl-1-pyrroline N-oxide (DMPO).
The tissue samples were mouse brain, hart, lung, liver, kidney, pancreas, muscle, skin, and whole blood, where various ESR spin adduct signals including DMPO-ascorbyl radical (AsA∗), DMPO-superoxide anion radical (OOH), and DMPO-hydrogen radical (H) signal were detected.
Postmortem changes in DMPO-AsA∗ and DMPO-OOH were observed in various tissues of mouse.
The signal peak of spin adduct was monitored until the 205th day postmortem.
DMPO-AsA∗ in liver (y=113.8–40.7 log (day), R1=-0.779, R2=0.6, P<.001) was found to linearly decrease with the logarithm of postmortem duration days.
Therefore, DMPO-AsA∗ signal may be suitable for detecting an oxidation stress tracer from tissue in comparison with other spin adduct signal on ESR spin trap method.
American Psychological Association (APA)
Ito, Shinobu& Mori, Tomohisa& Kanazawa, Hideko& Sawaguchi, Toshiko. 2011. Estimation of the Postmortem Duration of Mouse Tissue by Electron Spin Resonance Spectroscopy. Journal of Toxicology،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-512637
Modern Language Association (MLA)
Ito, Shinobu…[et al.]. Estimation of the Postmortem Duration of Mouse Tissue by Electron Spin Resonance Spectroscopy. Journal of Toxicology No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-512637
American Medical Association (AMA)
Ito, Shinobu& Mori, Tomohisa& Kanazawa, Hideko& Sawaguchi, Toshiko. Estimation of the Postmortem Duration of Mouse Tissue by Electron Spin Resonance Spectroscopy. Journal of Toxicology. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-512637
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-512637